Comparative analysis reveals the expansion of mitochondrial DNA control region containing unusually high G-C tandem repeat arrays in Nasonia vitripennis
Comparative analysis reveals the expansion of mitochondrial DNA control region containing unusually high G-C tandem repeat arrays in Nasonia vitripennis
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DOI：
10.1016/j.ijbiomac.2020.11.007
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发表时间：
2021-01-01
影响因子：
8.2
通讯作者：
Wang, Xu
中科院分区：
文献类型：
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作者：
Lin, Zi Jie;Wang, Xiaozhu;Wang, Xu
昆虫线粒体 DNA (mtDNA) 的范围为 14 至 19 kbp，大小差异归因于富含 AT 的控制区域。宝石黄蜂具有寄生生活方式，这可能会影响线粒体功能和进化。我们对 Nasonia 和外群物种的线粒体基因组进行了测序、组装和注释。 Nasonia 中的基因组成和顺序是保守的，但它们与其他寄生蜂的不同之处在于两个以前未报道过的大型反转事件。我们观察到吉拉蒂猪笼草和奥奈达猪笼草之间相对于核基因组和线粒体渗入的替换率要高得多，这与之前的研究一致。最引人注目的是，N. vitripennis mtDNA 具有极长的控制区（7665 bp），包含 29 个 217 bp 串联重复序列，并且可以折叠成超十字形结构。与其他线粒体中常见的串联重复序列相比，这些高拷贝重复序列高度保守（98.7% 序列同一性）、长度更长（约 8 Kb）、极其富含 GC（50.7%）和 CpG 丰富（CpG 百分比为 19.4%，编码区为 1.1%），导致 23 kbp 的 mtDNA 超出了昆虫的典型大小范围。这些 N. vitripennis 特异性线粒体重复序列与昆虫线粒体中的任何已知序列无关。它们的进化起源和功能后果值得进一步研究。 (C) 2020 Elsevier B.V. 保留所有权利。
Insect mitochondrial DNA (mtDNA) ranges from 14 to 19 kbp, and the size difference is attributed to the AT-rich control region. Jewel wasps have a parasitoid lifestyle, which may affect mitochondria function and evolution. We sequenced, assembled, and annotated mitochondrial genomes in Nasonia and outgroup species. Gene composition and order are conserved within Nasonia, but they differ from other parasitoids by two large inversion events that were not reported before. We observed a much higher substitution rate relative to the nuclear genome and mitochondrial introgression between N. giratilli and N. oneida, which is consistent with previous studies. Most strikingly, N. vitripennis mtDNA has an extremely long control region (7665 bp), containing twenty-nine 217 bp tandem repeats and can fold into a super-cruciform structure. In contrast to tandem repeats commonly found in other mitochondria, these high-copy repeats are highly conserved (98.7% sequence identity), much longer in length (approximately 8 Kb), extremely GC-rich (50.7%), and CpG-rich (percent CpG 19.4% vs. 1.1% in coding region), resulting in a 23 kbp mtDNA beyond the typical size range in insects. These N. vitripennis-specific mitochondrial repeats are not related to any known sequences in insect mitochondria. Their evolutionary origin and functional consequences warrant further investigations. (C) 2020 Elsevier B.V. All rights reserved.