Determining Surface Plasmon Resonance Response Factors for Deposition onto Three-Dimensional Surfaces.
Determining Surface Plasmon Resonance Response Factors for Deposition onto Three-Dimensional Surfaces.
复制标题
确定三维表面沉积的表面等离子共振响应因子。
DOI：
10.1016/j.ces.2006.12.014
复制
发表时间：
2007
影响因子：
4.7
通讯作者：
Roper,DKeith
中科院分区：
文献类型：
--
作者：
Roper,DKeith
配体/受体结合的固有吸附速率已通过表面等离子体共振（SPR）使用蛋白质或较小分子在平面表面上沉积的响应因子来测量。在这项研究中，SPR响应因子和有效折射率的广义表达式开发到3-D表面上的分析物吸附率的测量。该表达式是专门为两个限制的情况下，直接的实际利益和广泛的适用性：分析物沉积到一个均匀的各向异性多孔介质和沉积到紧密堆积的固体球体附近的传感器表面。这些新的方程指定介质容量，特征尺寸和分析物浓度，是必要的，以获得可识别的响应，从各向异性多孔介质或色谱树脂的相互作用。这些事态发展说明，通过比较平面表面，多孔介质和吸附球上的腺病毒5型的响应因子。
Intrinsic sorption rates of ligand/receptor binding have been measured by surface plasmon resonance (SPR) using response factors for deposition of proteins or smaller molecules on planar surfaces. In this study, generalized expressions for SPR response factor and effective refractive index are developed to measure rates of analyte sorption onto 3-D surfaces. The expressions are specialized for two limiting cases of immediate practical interest and broad applicability: analyte deposition onto a homogeneous anisotropic porous media and deposition onto close-packed solid spheres adjacent to the sensor surface. These new equations specify media capacity, characteristic size and analyte concentration that are necessary to obtain identifiable responses from interaction with anisotropic porous media or chromatographic resin. These developments are illustrated by comparing response factors for Adenovirus type 5 on planar surfaces, porous media and adsorptive spheres.