The SNPs in myoD gene from normal muscle developing individuals have no effect on muscle mass
The SNPs in myoD gene from normal muscle developing individuals have no effect on muscle mass
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正常肌肉发育个体的 myoD 基因中的 SNP 对肌肉质量没有影响
DOI：
10.1186/s12863-019-0772-6
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发表时间：
2019-09-02
期刊：
影响因子：
2.9
通讯作者：
Mo, Delin
中科院分区：
文献类型：
--
作者：
Ding, Suying;Nie, Yaping;Mo, Delin
背景肌源性分化1(MyoD)是调控肌肉特异性基因转录的重要主开关。强迫表达myoD可以诱导多个细胞系分化为成肌细胞，然后分化并融合成肌管。猪是最重要的供肉牲畜之一，分为瘦肉型、肥胖型和小型猪三大类。然而，不同品种猪之间肌肉质量差异的机制仍不清楚。考虑到MyoD在肌肉发育中的重要作用，肌肉质量的差异是否由其单核苷酸多态(SNPs)引起，这是猪品种间在DNA水平上的主要差异，目前仍有待研究。结果在本研究中，我们确定了猪MyoD调控区的位置，包括近端调控区(PRR)、远端调控区(DRR)和核心增强子(CE)区。在瘦肉型、肥胖型和小型猪群体中发现了8个位于调控区的SNPs和6个位于基因体区的SNPs。然而，这些SNP对其时间表达和转录活性没有影响，这可能导致出生后肌肉质量的差异。此外，从两栖动物到哺乳动物的myoD克隆的过表达可以促进NIH3T3成纤维细胞的肌肉生成，这些克隆的基因同源性从68%到84%不等。结论不同猪群体的myoD基因核苷酸变异对肌肉质量没有影响，提示myoD的功能不仅在不同的猪品种之间高度保守，而且在不同的物种之间也高度保守。因此，在肌肉发育正常的猪群体中发现影响肌肉质量的SNPs是徒劳的。
Background Myogenic Differentiation 1 (MyoD) is a crucial master switch in regulating muscle-specific gene transcription. Forced expression of myoD is equipped to induce several cell lineages into myoblast, which then differentiate and fuse into myotube. Pig is one of the most significant livestock supplying meat, and has been classified into lean, fat and miniature pig breeds. However, the mechanisms underlying muscle mass variation among different pig breeds have remained unclear. Considering the important effect of MyoD on muscle development, it remains to be investigated whether the difference in muscle mass is caused by its single nucleotide polymorphisms (SNPs) which are the major differences among pig breeds at DNA level. Results In this study, we identified the locations of porcine myoD regulatory regions including proximal regulatory region (PRR), distal regulatory region (DRR), and core enhancer (CE) region. There are 8 SNPs in the regulatory regions and 6 SNPs in gene body region, which were identified from lean, fat and miniature pig populations. However, these SNPs have no effects on its temporal expression and transcriptional activity which might lead to the distinction in postnatal muscle mass. In addition, overexpression of myoD clones across from amphibious to mammals including xenopus tropicalis, chicken, mouse and pig whose gene identities vary from 68 to 84%, could promote myogenesis in NIH3T3 fibroblasts cells. Conclusions These results proved that myoD nucleotide variations from different pig populations have no effect on muscle mass, suggesting that the function of myoD is highly conserved not only among different pig breeds, but also across different species. Thus, it would be futile to discover SNPs affecting muscle mass in pig populations with normal muscle development.