Increased cardiovascular risk in association with chronic airflow obstruction among premenopausal rural women of India who cook exclusively with biomass
Increased cardiovascular risk in association with chronic airflow obstruction among premenopausal rural women of India who cook exclusively with biomass
复制标题
DOI：
10.1007/s11869-012-0173-8
复制
发表时间：
2013-03-01
影响因子：
5.1
通讯作者：
Chowdhury, Saswati
中科院分区：
文献类型：
--
作者：
Dutta, Anindita;Ray, Manas Ranjan;Chowdhury, Saswati
我们的目的是比较患有或不患有慢性阻塞性肺病（COPD）的使用生物质的女性的心血管风险。为此目的，总共招募了 22 名使用生物质的患有 COPD 的已婚女性和 24 名肺功能正常的匹配对照者。使用流式细胞术测定血小板 P-选择素 (P-sel) 表达和血小板白细胞聚集。通过聚集计测量胶原引起的血小板聚集。血浆中可溶性P-选择素（sP-sel）、肿瘤坏死因子-α（TNF-α）、白细胞介素-8、-6、-10（IL-8、IL-6、IL-10）、中性粒细胞激活蛋白-2（NAP-2）、C反应蛋白（CRP）、氧化低密度脂蛋白（oxLDL）通过以下方法测定 酶联免疫吸附测定。通过流式细胞术测量白细胞产生的活性氧（ROS），通过分光光度法测量红细胞超氧化物歧化酶（SOD）含量。通过实时气溶胶监测仪测量室内空气中直径小于2.5μm的颗粒物（PM2.5）。与对照组相比，患有 COPD 的生物质使用者血小板 P-选择素表达增加，sP-sel、oxLDL、TNF-α、IL-8、IL-6、NAP-2、CRP 水平升高，IL-10 降低，循环血小板中性粒细胞 (p < 0.0001) 和血小板单核细胞 (p < 0.0001) 聚集体增加。患有 COPD 的女性中，ROS 生成量增加了 19.5%，而 SOD 减少了 32%。生物质烟雾诱发的慢性阻塞性肺病通过氧化应激、血小板活化和炎症与过度的心血管风险相关。
We aimed to compare the cardiovascular risk in biomass-using women with or without chronic obstructive pulmonary disease (COPD). A total of 22 biomass-using married women with COPD and 24 matched controls with normal lung function were enrolled for this purpose. Platelet P-selectin (P-sel) expression and platelet-leukocyte aggregation were determined using flow cytometry. Platelet aggregation by collagen was measured by aggregometer. Soluble P-selectin (sP-sel), tumor necrosis factor-alpha (TNF-alpha), interleukin-8, -6, -10 (IL-8, IL-6, IL-10), neutrophil-activating protein-2 (NAP-2), C-reactive protein (CRP), oxidized low density lipoprotein (oxLDL) in plasma were measured by enzyme-linked immunosorbent assay. Generation of reactive oxygen species (ROS) by leukocytes was measured by flow cytometry, and erythrocyte content of superoxide dismutase (SOD) was measured by spectrophotometry. Particulate matter with a diameter of less than 2.5 mu m (PM2.5) in indoor air was measured by real-time aerosol monitor. Compared with control, biomass users with COPD had increased expression of platelet P-selectin, elevated levels of sP-sel, oxLDL, TNF-alpha, IL-8, IL-6, NAP-2, CRP, lowered IL-10 and more circulating platelet-neutrophil (p < 0.0001) and platelet-monocyte (p < 0.0001) aggregates. ROS generation was increased by 19.5% while SOD was depleted by 32% in women with COPD. Biomass smoke-induced COPD is associated with excess cardiovascular risk via oxidative stress, platelet activation, and inflammation.