Colocalization of transcription and translation within cytoplasmic poxvirus factories coordinates viral expression and subjugates host functions
Colocalization of transcription and translation within cytoplasmic poxvirus factories coordinates viral expression and subjugates host functions
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DOI：
10.1016/j.chom.2007.08.005
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发表时间：
2007-10-01
影响因子：
30.3
通讯作者：
Moss, Bernard
中科院分区：
文献类型：
--
作者：
Katsafanas, George C.;Moss, Bernard
痘病毒是大型 DNA 病毒，包括人类天花和痘苗病毒的病原体。痘病毒在称为 DNA 工厂的细胞质灶中复制。在这里，我们发现病毒编码的转录因子、病毒mRNA、细胞RNA结合蛋白异二聚体G3BP/Caprin-1 (p137)、翻译起始因子elF4E和elF4G以及核糖体蛋白集中在细胞质DNA工厂的相同子结构域中。此外，用表达与青色或黄色荧光蛋白融合的病毒核心蛋白的两种重组牛痘病毒共感染的细胞显示出单独的青色和黄色工厂，表明每个工厂由单个基因组形成，并且是转录和翻译以及DNA复制的位点。在工厂内劫持宿主翻译装置可能会提高病毒复制的效率，并有助于抑制宿主蛋白质的合成，从而促进痘病毒征服细胞。
Poxviruses are large DNA viruses that include the causal agent of human smallpox and vaccinia virus. Poxviruses replicate in cytoplasmic foci known as DNA factories. Here we show that a virus-encoded transcription factor, viral mRNA, cellular RNA-binding protein heterodimer G3BP/Caprin-1 (p137), translation initiation factors elF4E and elF4G, and ribosomal proteins are concentrated in the same sub-domains of cytoplasmic DNA factories. Furthermore, a cell coinfected with two recombinant vaccinia viruses expressing a virus core protein fused to cyan or yellow fluorescent protein displayed separate cyan and yellow factories, indicating that each factory formed from a single genome and was the site of transcription and translation as well as DNA replication. Hijacking of the host translation apparatus within the factory likely enhances the efficiency of virus replication and contributes to the suppression of host protein synthesis, thereby facilitating poxvirus subjugation of the cell.