Olivary climbing fiber alterations in PN40 rat cerebellum following postnatal ethanol exposure.
Olivary climbing fiber alterations in PN40 rat cerebellum following postnatal ethanol exposure.
复制标题
DOI：
10.1016/j.brainres.2011.01.028
复制
发表时间：
2011-03-10
期刊：
影响因子：
2.9
通讯作者：
Light KE
中科院分区：
文献类型：
--
作者：
Pierce DR;Hayar A;Williams DK;Light KE
已知在大鼠出生后4-6天（PN）期间的发育性乙醇暴露会导致小脑浦肯野细胞的显著损失。目前尚不清楚存活下来的神经元在继续发育过程中会发生什么。本研究旨在量化小脑回路成熟时橄榄树攀爬纤维与浦肯野细胞之间的相互作用。大鼠幼仔通过PN4、PN4-6或PN7-9灌胃给予每日剂量的乙醇（4.5 g/kg体重）。用共聚焦显微镜观察攀爬纤维与浦肯野细胞的相互作用。用calbinin - d28k抗体（用于观察浦肯野细胞）和水泡型谷氨酸转运蛋白2 （VGluT2，用于观察攀爬纤维）对小脑中部切片进行染色。从小叶1处获得共聚焦z叠图像，用Imaris软件进行分析，量化两种抗体的染色情况。VGluT2免疫染色显著降低，这与突触完整性和每个浦肯野细胞突触数量的改变有关，仅PN4一次暴露就足以引起这种改变。之前，我们在分析PN14时发现了攀爬纤维神经支配的类似缺陷。目前的研究证实，这些改变是持续的，并进一步确定了突触密度的降低以及小脑皮层分子层的一般形态的改变，这是酗酒乙醇暴露的结果。
Developmental ethanol exposure in rats during postnatal days (PN) 4–6 is known to cause significant loss of cerebellar Purkinje cells. It is not known what happens to the surviving neurons as they continue to develop. This study was designed to quantify the interactions between the olivary climbing fibers and the Purkinje cells when the cerebellar circuits have matured. Rat pups were treated with a daily dose of ethanol (4.5 g/kg body weight) delivered by intragastric intubation on PN4, PN4-6, or PN7-9. The interactions between climbing fibers and Purkinje cells were examined on PN40 using confocal microscopy. Mid-vermal cerebellar sections were stained with antibodies to calbindin-D28k (to visualize Purkinje cells) and vesicular glutamate transporter 2 (VGluT2, to visualize climbing fibers). Confocal z-stack images were obtained from Lobule 1 and analyzed with Imaris software to quantify the staining of the two antibodies. The VGluT2 immunostaining was significantly reduced and this was associated with alterations in the synaptic integrity, and synaptic number per Purkinje cell with only a single exposure on PN4 enough to cause the alterations. Previously, we demonstrated similar deficits in climbing fiber innervation when analyzed on PN14. The present study confirms that these alterations are sustained and further identifies the decreased synaptic density as well as alterations to the general morphology of the molecular layer of the cerebellar cortex that are the result of the binge ethanol exposure.
登录
查看更多内容
影响因子：
3.3
作者：
Kato, Y;Takatsuki, K;Kirino, Y
通讯作者：
Kirino, Y
影响因子：
3.3
作者：
Marvizon, J. C. G.;Perez, O. A.;Todd, A. J.
通讯作者：
Todd, A. J.
DOI：
10.1111/j.1530-0277.1993.tb00808.x
发表时间：
1993-06-01
影响因子：
3.2
作者：
HAMRE, KM;WEST, JR
通讯作者：
WEST, JR
影响因子：
3.3
作者：
Hioki, H;Fujiyama, F;Kaneko, T
通讯作者：
Kaneko, T
DOI：
10.1016/j.molbrainres.2004.06.034
发表时间：
2004-10-22
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Ge, Y;Belcher, SA;Light, KE
通讯作者：
Light, KE