RNA SPLICE JUNCTIONS OF DIFFERENT CLASSES OF EUKARYOTES - SEQUENCE STATISTICS AND FUNCTIONAL IMPLICATIONS IN GENE-EXPRESSION
RNA SPLICE JUNCTIONS OF DIFFERENT CLASSES OF EUKARYOTES - SEQUENCE STATISTICS AND FUNCTIONAL IMPLICATIONS IN GENE-EXPRESSION
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DOI：
10.1093/nar/15.17.7155
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发表时间：
1987-09-11
影响因子：
14.9
通讯作者：
SENAPATHY, P
中科院分区：
文献类型：
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作者：
SHAPIRO, MB;SENAPATHY, P
使用 GENBANK 数据库对真核蛋白编码基因的 RNA 剪接点序列进行了系统分析。不同类别生物体剪接位点周围高度保守区域的核苷酸频率彼此非常一致。 3'剪接位点不含AG或5'剪接位点不含GT的罕见剪接点之间存在惊人的相似性，表明存在识别它们的特殊机制，并且这些独特的信号可能涉及关键的基因调控事件和分化。开发了一种方法来预测裸序列中的潜在外显子，使用基于核苷酸权重表的评分和排名方案。该方法用于查找选定的已知基因中的大多数外显子，并预测可用于替代剪接情况的潜在新外显子。
A systematic analysis of the RNA splice junction sequences of eukaryotic protein coding genes was carried out using the GENBANK databank. Nucleotide frequencies obtained for the highly conserved regions around the splice sites for different categories of organisms closely agree with each other. A striking similarity among the rare splice junctions which do not contain AG at the 3′ splice site or GT at the 5′ splice site indicates the existence of special mechanisms to recognize them, and that these unique signals may be involved in crucial gene-regulation events and in differentiation. A method was developed to predict potential exons in a bare sequence, using a scoring and ranking scheme based on nucleotide weight tables. This method was used to find a majority of the exons in selected known genes, and also predicted potential new exons which may be used in alternative splicing situations.