Charge detection mass spectrometry for the analysis of viruses and virus-like particles.
Charge detection mass spectrometry for the analysis of viruses and virus-like particles.
复制标题
用于分析病毒和病毒样颗粒的电荷检测质谱法。
DOI：
10.1042/ebc20220101
复制
发表时间：
2023
影响因子：
6.4
通讯作者：
Jarrold,MartinF
中科院分区：
文献类型：
--
作者：
Miller,LohraM;Jarrold,MartinF
异质性通常将传统的质谱学限制在分子质量小于一兆吨左右。作为一种单粒子技术，电荷检测质谱仪(CDMS)克服了这一局限。在CDMS中，同时测量单个离子的质量电荷比(m/z)和电荷，给出每个离子的直接质量测量。最近的应用包括病毒、病毒样颗粒、疫苗、高度糖基化的蛋白质和基因治疗载体的分析。
Heterogeneity usually restricts conventional mass spectrometry to molecular weights less than around a megadalton. As a single-particle technique, charge detection mass spectrometry (CDMS) overcomes this limitation. In CDMS, the mass-to-charge (m/z) ratio and charge are measured simultaneously for individual ions, giving a direct mass measurement for each ion. Recent applications include the analysis of viruses, virus-like particles, vaccines, heavily glycosylated proteins, and gene therapy vectors.