Neuroprotective effects of Sophora secundiflora, Sophora tomentosa leaves and formononetin on scopolamine-induced dementia
Neuroprotective effects of Sophora secundiflora, Sophora tomentosa leaves and formononetin on scopolamine-induced dementia
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DOI：
10.1080/14786419.2020.1795853
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发表时间：
2020-07-21
影响因子：
2.2
通讯作者：
Singab, Abdel Nasser B.
中科院分区：
文献类型：
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作者：
Aly, Shaza H.;Elissawy, Ahmed M.;Singab, Abdel Nasser B.
从苦豆叶乙酸乙酯部位分离得到5个黄酮类化合物：芒柄花素(1)、5-羟基-4‘-甲氧基黄酮(2)、染料木素(3)、5-hydroxy-8-(1-hydroxy-1-methyl-ethyl)-2-(4-hydroxyphenyl)-4H-furo-[2，3-h]-色满-4-酮(4)和橙花素(5)。此外，还进行了乙酸乙酯馏分的LC-ESI-MS/MS分析。亚丁二氟叶导致了18种化合物的初步鉴定。Formononetin，S.tomentosaand S.对芒柄花叶乙醇提取物的神经保护活性进行了活体评价，其中芒柄花素和芒柄花素的活性最高。托马斯通能显著降低乙酰胆碱酯酶(AChE)活性，升高脑内乙酰胆碱(Ach)和谷胱甘肽(GSH)水平，降低多巴胺(DA)、去甲肾上腺素(NA)和丙二醛(MDA)水平，具有良好的神经保护作用。二花叶醇提物不能降低脑组织AChE酶活性、DA和NA含量。
Five flavonoids were isolated from the ethyl acetate fraction of leaves ofSophora secundiflora; formononetin(1), 5-hydroxy-4'-methoxyflavone(2), genistein(3), 5-hydroxy-8-(1-hydroxy-1-methyl-ethyl)-2-(4-hydroxyphenyl)-4H-furo-[2, 3-h]-chromen-4-one(4)and ononin(5). Additionally, LC-ESI-MS/MS analysis of the ethyl acetate fraction ofS. secundifloraleaves had led to tentative identification of eighteen compounds. Formononetin,S. tomentosaandS. secundifloraleaves methanolic extract were evaluatedin vivofor their neuroprotective activity where formononetin andS. tomentosashowed promising neuroprotective activity with reduction in acetylcholine esterase (AchE) enzyme activity and elevation of acetylcholine (Ach) and glutathione(GSH) brain levels and attenuation of dopamine (DA), nor-adrenaline (NA) and malonedialdehyde (MDA) brain level significantly, HoweverS. secundifloraleaves methanolic extract didn't attenuate the AchE enzyme activity, DA and NA brain levels.[GRAPHICS]