Catellatospora vulcania sp nov and Catellatospora paridis sp nov., two novel actinobacteria isolated from volcanic sediment and the rhizosphere of Paris polyphylla
Catellatospora vulcania sp nov and Catellatospora paridis sp nov., two novel actinobacteria isolated from volcanic sediment and the rhizosphere of Paris polyphylla
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DOI：
10.1007/s10482-015-0608-y
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发表时间：
2016-01-01
影响因子：
2.6
通讯作者：
Xiang, Wensheng
中科院分区：
文献类型：
--
作者：
Jia, Feiyu;Guo, Siyu;Xiang, Wensheng
从吉林省和黑龙江省的火山沉积物和多叶巴黎根际土壤中分离到两株放线菌，命名为NEAU-JM 1（T）和NEAU-CL 2（T）。进行了多相研究，以建立这些菌株的分类位置。16 S rRNA基因序列分析表明，两个新分离物的16 S rRNA基因序列相似性为99.5%。基于16 S rRNA基因序列的系统发育分析表明，菌株NEAU-JM 1（T）与Catellatospora methionotrophica JCM 7543（T）亲缘关系较近。（99.4%序列相似性），考克斯链孢菌DSM 44901（T）（99.4%）、柠檬杯孢菌DSM 44097（T）（99.3%）和长颈杯孢菌JCM 12950（T）（99.2%）; citrea DSM 44097（T），C.甲硫氨酸营养型（MethionotrophicaJCM 7543（T））、C. chokoriensis JCM 12950（T）和C. coxensis DSM 44901（T）（99.2%）。而菌株NEAU-JM 1（T）和NEAU-CL 2（T）之间的DNA-DNA杂交值为62.2%，与其亲缘关系较近的菌株之间的杂交值也低于70%。参考表型特征、系统发育数据和DNA-DNA杂交结果，可以将这两个菌株彼此区分开来，并区分出它们的亲缘关系。因此，菌株NEAU-JM 1（T）和NEAU-CL 2（T）可以被认为是Catellatospora属的两个新种，并建议将其命名为Catellatospora vulcania sp. nov.和Catellatospora paranum sp. nov。模式菌株分别为NEAU-JM 1（T）（=CGMCC 4.7174（T）= JCM 30054（T））和NEAU-CL 2（T）（=CGMCC 4.7236（T）= DSM 100519（T））。
Two novel actinobacteria, designated strains NEAU-JM1(T) and NEAU-CL2(T), were isolated from volcanic sediment and the rhizosphere soil of Paris polyphylla, respectively, collected from Jiling and Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic positions of these strains. The 16S rRNA gene sequence analysis showed that the two novel isolates exhibit 99.5 % 16S rRNA gene sequence similarity with each other. The phylogenetic analysis based on 16S rRNA gene sequence of strain NEAU-JM1(T) showed it to be closely related to Catellatospora methionotrophica JCM 7543(T) (99.4 % sequence similarity), Catellatospora coxensis DSM 44901(T) (99.4 %), Catellatospora citrea DSM 44097(T) (99.3 %) and Catellatospora chokoriensis JCM 12950(T) (99.2 %), and that of strain NEAU-CL2(T) to C. citrea DSM 44097(T) (99.4 %), C. methionotrophica JCM 7543(T) (99.3 %), C. chokoriensis JCM 12950(T) (99.3 %) and C. coxensis DSM 44901(T) (99.2 %). However, the DNA-DNA hybridization value between strains NEAU-JM1(T) and NEAU-CL2(T) was 62.2 %, and the values between the two strains and their close phylogenetic relatives were also below 70 %. With reference to phenotypic characteristics, phylogenetic data and DNA-DNA hybridization results, the two strains can be distinguished from each other and their close phylogenetic relatives. Thus, strains NEAU-JM1(T) and NEAU-CL2(T) can be concluded to represent two novel species of the genus Catellatospora, for which the names Catellatospora vulcania sp. nov. and Catellatospora paridis sp. nov. are proposed. The type strains are NEAU-JM1(T) (=CGMCC 4.7174(T) = JCM 30054(T)) and NEAU-CL2(T) (=CGMCC 4.7236(T) = DSM 100519(T)), respectively.