Temporal regulation of morphogenetic events in Saccharomyces cerevisiae.
Temporal regulation of morphogenetic events in Saccharomyces cerevisiae.
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DOI：
10.1091/mbc.e18-03-0188
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发表时间：
2018-08-15
影响因子：
3.3
通讯作者：
Lew DJ
中科院分区：
文献类型：
--
作者：
Lai H;Chiou JG;Zhurikhina A;Zyla TR;Tsygankov D;Lew DJ
真菌的尖端生长涉及高度极化的分泌和生长尖端细胞壁的修饰。启动极化生长的遗传要求可能是最好的理解模式芽殖酵母酿酒酵母。一旦细胞通过G1周期蛋白/周期蛋白依赖性激酶（CDK）复合物的激活进入细胞周期，极性调节因子Cdc42就集中在假定的芽位点，肌动蛋白电缆朝向该位点，隔素丝在极性位点周围组装成一个环。几分钟后，芽发芽了。在这里，我们研究了在单细胞水平上调节这些事件发生时间的机制。相对于极性的建立，Septin的招募被延迟，我们的研究结果表明cdk依赖的Septin“启动”促进了Cdc42对Septin的招募。相对于极化分泌的开始，芽的出现是延迟的，我们的研究结果表明，延迟反映了细胞充分削弱细胞壁以发芽所需的时间。Rho1被Rom2激活大约发生在芽出的时候，可能是对局部细胞壁减弱的反应。本报告揭示了芽殖酵母形态发生事件的调控机制。
Tip growth in fungi involves highly polarized secretion and modification of the cell wall at the growing tip. The genetic requirements for initiating polarized growth are perhaps best understood for the model budding yeast Saccharomyces cerevisiae. Once the cell is committed to enter the cell cycle by activation of G1 cyclin/cyclin-dependent kinase (CDK) complexes, the polarity regulator Cdc42 becomes concentrated at the presumptive bud site, actin cables are oriented toward that site, and septin filaments assemble into a ring around the polarity site. Several minutes later, the bud emerges. Here, we investigated the mechanisms that regulate the timing of these events at the single-cell level. Septin recruitment was delayed relative to polarity establishment, and our findings suggest that a CDK-dependent septin “priming” facilitates septin recruitment by Cdc42. Bud emergence was delayed relative to the initiation of polarized secretion, and our findings suggest that the delay reflects the time needed to weaken the cell wall sufficiently for the cell to bud. Rho1 activation by Rom2 occurred at around the time of bud emergence, perhaps in response to local cell-wall weakening. This report reveals regulatory mechanisms underlying the morphogenetic events in the budding yeast.