Identification and expression analysis of PIN genes in rice
Identification and expression analysis of PIN genes in rice
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DOI：
10.1016/j.plantsci.2010.02.018
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发表时间：
2010-05-01
期刊：
影响因子：
5.2
通讯作者：
Ito, Yukihiro
中科院分区：
文献类型：
--
作者：
Miyashita, Yui;Takasugi, Tomoaki;Ito, Yukihiro
我们在水稻中鉴定了12个编码植物激素生长素外排载体蛋白的PIN基因。植物遗传学分析表明，11个基因与拟南芥PIN基因属于分支，1个基因在拟南芥中没有同源性。RT-PCR分析表明，10个PIN基因在不同器官中的表达依赖于基因。在所检查的器官中未检测到0 sPIN 3b的表达。OsPIN 1b和OsPIN 1d在序列和表达模式上都非常相似，在茎尖中，OsPIN 1b/1d在幼叶维管组织发育的区域表达。OsPIN 2. OsPIN 3a和OsPIN 5a在整个茎尖中表达，在最外层细胞层中略有偏好。这些结果表明，水稻有几个不同的成员的PIN基因具有不同的表达模式，并建议极性生长素运输介导的PIN基因在水稻的每个器官中的不同组合。(C)2010爱思唯尔爱尔兰有限公司版权所有。
We identified 12 PIN genes in rice which encode efflux carrier proteins of phytohormone auxin. Phytogenetic analysis showed that 11 genes were categorized into branches with Arabidopsis PIN genes and one had no close homologue in Arabidopsis. RT-PCR analysis showed expression of 10 PIN genes in various organs depending on genes. No expression of OsPIN3b was detected in the organs examined. OsPIN1b and OsPIN1d were very similar both in sequence and in expression pattern, and in the shoot apex, OsPIN1b/1d was expressed in regions where vascular tissues were developing in young leaf. OsPIN2. OsPIN3a and OsPIN5a were expressed in the entire shoot apex with slight preference in an outermost cell layer. These results indicate that rice has several distinct members of the PIN genes which have various expression patterns, and suggest that polar auxin transport is mediated by different combinations of the PIN genes in each organ in rice. (C) 2010 Elsevier Ireland Ltd. All rights reserved.