MODELS TO INTERPRET KINETIC DATA IN STABLE ISOTOPE TRACER STUDIES
MODELS TO INTERPRET KINETIC DATA IN STABLE ISOTOPE TRACER STUDIES
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DOI：
10.1152/ajpendo.1987.253.5.e551
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发表时间：
1987-11-01
影响因子：
--
通讯作者：
NOSADINI, R
中科院分区：
文献类型：
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作者：
COBELLI, C;TOFFOLO, G;NOSADINI, R
与“失重”放射性示踪剂相反，稳定同位素示踪剂具有不可忽略的质量，并且天然存在于系统中，并且测量的变量是两种同位素物质的比率。这些特征不允许使用与放射性示踪剂方法直接类比的稳定同位素示踪剂数据分析，即使这种做法很常见。在这项研究中，我们提出了动力学变量，模型和测量的稳定同位素示踪数据的分析和解释。假设和数学技术建模的数据时，扰动是nonneligible和可忽略不计的讨论。重点放在稳定同位素示踪曲线和房室和非房室建模方法的作用，其解释。放射性比活度和稳定同位素富集之间的假定和常用的类比被证明是不正确的。我们表明，比活度的适当的类似物是示踪剂的tracee摩尔比。这个变量不是一个可直接测量的变量，但可以推导出一个公式，允许从数据中计算它。本文提出了一种用于重建内源性合成的浓度分量在血液中的时间过程的方法。这允许在使用失重示踪剂的情况下测量扰动的程度。特别注意的是数据分析源自多示踪剂实验，一个配置必要的研究更复杂的系统，例如，相互作用基质的动力学。
In contrast to "weightless" radioactive tracers, stable isotope tracers have nonnegligible mass and are naturally present in the system, and the measured variable is a ratio of two isotopic species. These features do not allow stable isotopic tracer data analysis using straightforward analogy with radioactive tracer approaches, even though this practice is common. In this study, we present kinetic variables, models, and measurements for the analysis and interpretation of stable isotope tracer data. Assumptions and mathematical techniques for modeling the data when perturbation is both nonneligible and negligible are discussed. Emphasis is placed stable isotope tracer curve and on the role of compartmental and noncompartmental modeling approaches for its interpretation. A presumed and commonly used analogy between the radioactive specific activity and stable isotopic enrichment is shown to be incorrect. We show that the proper analogue of specific activity is the tracer-to-tracee molar ratio. This variable is not a directly measurable one, but a formula is derived that allows its computation from the data. A method for reconstructing the time course in blood of the concentration component due to endogenous synthesis is presented. This allows measurement of the extent of the perturbation in the case where a nonweightless tracer is use. Special attention is given to data analysis originating from a multiple tracer experiment, a configuration necessary for studying more complex systems, e.g., the kinetics of interacting substrates.