Human parainfluenza virus type I (HPIV1) vaccine candidates designed by reverse genetics are attenuated and efficacious in African green monkeys
Human parainfluenza virus type I (HPIV1) vaccine candidates designed by reverse genetics are attenuated and efficacious in African green monkeys
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DOI：
10.1016/j.vaccine.2005.04.035
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发表时间：
2005-09-07
期刊：
影响因子：
5.5
通讯作者：
Skiadopoulos, MH
中科院分区：
文献类型：
--
作者：
Bartlett, EJ;Amaro-Carambot, E;Skiadopoulos, MH
在非洲绿猴（AGMs）中评估了一组重组人副流感病毒I型减毒活疫苗候选物的衰减、免疫原性和保护效果。P/C和L基因的温敏（ts）和非温敏（att）突变被单独或以各种组合引入到rHPIV1中，包括本研究发现的C- r84g和HNT553A突变，以及之前发现的C- f170s、L- y942a和L- l992c突变。rHPIV1候选疫苗在agm中表现出衰减谱。一种遗传和表型稳定的候选疫苗rC(R84G/F170S)L（Y942A/L992C）在AGMs中减毒有效，是一种有前途的鼻内减毒活疫苗，适合临床评价。Elsevier Ltd.出版。
A set of recombinant, live attenuated human parainfluenza virus type I (rHPIV1) vaccine candidates was evaluated for attenuation, immunogenicity, and protective efficacy in African green monkeys (AGMs). Temperature sensitive (ts) and non-ts attenuating (att) mutations in the P/C and L genes were introduced individually or in various combinations into rHPIV1, including the C-R84G and HNT553A mutations identified in the present work and the C-F170S, L-Y942A, and L-L992C mutations identified previously. The rHPIV1 vaccine candidates exhibited a spectrum of attenuation in AGMs. One genetically and phenotypically stable vaccine candidate, rC(R84G/F170S)L(Y942A/L992C), was attenuated and efficacious in AGMs and is a promising live attenuated intranasal HPIV I vaccine candidate suitable for clinical evaluation. Published by Elsevier Ltd.