Ectopic Expression of DREB Transcription Factor, AtDREB1A, Confers Tolerance to Drought in Transgenic Salvia miltiorrhiza
Ectopic Expression of DREB Transcription Factor, AtDREB1A, Confers Tolerance to Drought in Transgenic Salvia miltiorrhiza
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DREB转录因子AtDREB1A的异位表达赋予转基因丹参耐旱性
DOI：
10.1093/pcp/pcw084
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发表时间：
2016
影响因子：
4.9
通讯作者：
Zhang Yong
中科院分区：
文献类型：
--
作者：
Wei Tao;Deng Kejun;Liu Dongqing;Gao Yonghong;Liu Yu;Yang Meiling;Zhang Lipeng;Zheng Xuelian;Wang Chunguo;Song Wenqin;Chen Chengbin;Zhang Yong
干旱比任何其他类型的环境压力对作物生产力的影响都更大。转录因子在调控植物非生物胁迫反应中起着至关重要的作用。将编码逆境诱导因子的拟南芥DREB1A/CBF3基因导入丹参。AtDREB1A的异源表达提高了植株的抗旱性，在干旱胁迫下，转基因株系具有较高的相对含水量和Chl含量，表现出较高的光合速率。在干旱胁迫下，转AtDREB1A基因植株的丙二醛(MDA)含量较低，而超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性较高。特别是，在胁迫诱导的RD29A启动子控制下异位表达AtDREB1A的植株比转P35S：：AtDREB1A的植株表现出更强的抗旱性，没有生长抑制或表型异常。野生型和pRD29A：：AtDREB1A转基因植株干旱胁迫后的差异基因表达谱显示，与胁迫响应、光合作用、信号转导、碳水化合物代谢和蛋白质保护相关的各种基因的表达水平在转基因植株中显著高于野生型和pRD29A：：AtDREB1A转基因植株。此外，在AtDREB1a转基因动物中，丹酚酸和丹参酮的含量显著增加。丹参和相关生物合成途径中的大多数基因表达上调。综上所述，这些结果表明，诱导Tf的表达可以有效地调控胁迫反应途径中的多个基因，显著提高植物对非生物胁迫的抗性。我们的结果还表明，对转铁蛋白的遗传操作可以通过调节相关途径中的基因来提高有价值的次生代谢物的产生。
Drought decreases crop productivity more than any other type of environmental stress. Transcription factors (TFs) play crucial roles in regulating plant abiotic stress responses. TheArabidopsis thalianagene DREB1A/CBF3, encoding a stress-inducible TF, was introduced intoSalvia miltiorrhiza. Ectopic expression of AtDREB1A resulted in increased drought tolerance, and transgenic lines had higher relative water content and Chl content, and exhibited an increased photosynthetic rate when subjected to drought stress. AtDREB1A transgenic plants generally displayed lower malondialdehyde (MDA), but higher superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities under drought stress. In particular, plants with ectopic AtDREB1A expression under the control of the stress-induced RD29A promoter exhibited more tolerance to drought compared with p35S::AtDREB1A transgenic plants, without growth inhibition or phenotypic aberrations. Differential gene expression profiling of wild-type and pRD29A::AtDREB1A transgenic plants following drought stress revealed that the expression levels of various genes associated with the stress response, photosynthesis, signaling, carbohydrate metabolism and protein protection were substantially higher in transgenic plants. In addition, the amount of salvianolic acids and tanshinones was significantly elevated in AtDREB1A transgenicS. miltiorrhizaroots, and most of the genes in the related biosynthetic pathways were up-regulated. Together, these results demonstrated that inducing the expression of a TF can effectively regulate multiple genes in the stress response pathways and significantly improve the resistance of plants to abiotic stresses. Our results also suggest that genetic manipulation of a TF can improve production of valuable secondary metabolites by regulating genes in associated pathways.