Cryopreservation of spermatozoa from cynomolgus monkeys (Macaca fascicularis).
Cryopreservation of spermatozoa from cynomolgus monkeys (Macaca fascicularis).
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DOI：
10.1530/jrf.0.1010273
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发表时间：
1994-07
期刊：
影响因子：
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通讯作者：
Tadashi Sankai;K. Terao;R. Yanagimachi;Fumiaki Cho;Yasuhiro Yoshikawa
中科院分区：
文献类型：
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作者：
Tadashi Sankai;K. Terao;R. Yanagimachi;Fumiaki Cho;Yasuhiro Yoshikawa
使用冻融的精子在体外使食蟹猴（Macaca fasciculis）的卵子受精。将精子冷冻在含有5%甘油的精液稀释培养基（TTE：Tes、Tris、蛋黄基）中平衡时间为30分钟后获得最佳精子存活率。尽管冻融的精子可以在体外使卵子受精，并且一些受精卵发育成囊胚或孵化的囊胚，但使用盐储存卵的精子生育力测定表明，冻融的精子在获能培养基中的寿命比新鲜的未冷冻的精子短。这可能归因于它们的质膜受损。
Fertilization of eggs from cynomolgus monkeys (Macaca fascicularis) was achieved in vitro using frozen-thawed spermatozoa. The best sperm survival rate was obtained after freezing spermatozoa in a semen-diluting medium (TTE: Tes, Tris, egg yolk base) containing 5% glycerol with 30 min equilibrium time. Although frozen-thawed spermatozoa could fertilize eggs in vitro and some of the fertilized eggs developed into blastocysts or hatched blastocysts, a sperm fertility assay using salt-stored eggs indicated that frozen-thawed spermatozoa had a shorter lifespan in capacitation medium than did fresh, unfrozen spermatozoa. This may be attributable to damage to their plasma membranes.