Central administration of the RFamide peptides, QRFP-26 and QRFP-43, increases high fat food intake in rats
Central administration of the RFamide peptides, QRFP-26 and QRFP-43, increases high fat food intake in rats
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DOI：
10.1016/j.peptides.2008.07.024
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发表时间：
2008-11-01
期刊：
影响因子：
3
通讯作者：
Bray, George A.
中科院分区：
文献类型：
--
作者：
Primeaux, Stefany D.;Blackmon, Christine;Bray, George A.
焦谷氨酰化精氨酸-苯丙氨酸酰胺肽 (QRFP) 在物种间高度保守，是 RFamide 相关肽家族的成员，其 C 末端有基序 Arg-Phe-NH2。 QRFP 的前体肽产生 26 个氨基酸肽 (QRFP-26) 和 43 个氨基酸肽 (QRFP-43)，两者均与 G 蛋白偶联受体 GPR103 结合。最近，QRFP 在大鼠、小鼠和人类中得到了表征，并据报道具有促进食欲的特性。在啮齿类动物中，前原 QRFP mRNA 在下丘脑内侧基底区域的局部区域表达，该区域与摄食行为有关。高脂肪饮食摄入量的增加会导致体重增加和肥胖。因此，当前的实验研究了 QRFP 给药对大鼠的影响以及高脂肪饮食对前 QRFP mRNA 和 GPR103 受体 mRNA 水平的影响。脑室内给予QRFP-26（3.0 nM，5.0 nM）和QRFP-43（1.0 nM，3.0 nM）剂量依赖性地增加高脂肪（55％脂肪）饮食的1小时、2小时和4小时累积摄入量，但不增加低脂肪（10％脂肪）饮食。在实验2中，测量了喂食高脂肪或低脂肪饮食21天的大鼠的下丘脑前QRFP原mRNA水平和GPR103受体mRNA水平。与低脂饮食的大鼠相比，高脂饮食的大鼠下丘脑腹内侧核/弓状核中的 Prepro-QRFP mRNA 显着增加，而 GPR103 mRNA 水平没有变化。这些研究结果表明，QRFP 是膳食脂肪摄入量的调节剂，并受到高脂肪饮食摄入量的影响。由爱思唯尔公司出版
Pyrogultamylated arginine-phenylalanineamide peptide (QRFP) is strongly conserved across species and is a member of the family of RFamide-related peptides, with the motif Arg-Phe-NH2 at the C-terminal end. The precursor peptide for QRFP generates a 26-amino acid peptide (QRFP-26) and a 43-amino acid peptide (QRFP-43), both of which bind to the G protein-coupled receptor, GPR103. Recently, QRFP has been characterized in rats, mice and humans and has been reported to have orexigenic properties. In rodents, prepro-QRFP mRNA is expressed in localized regions of the mediobasal hypothalamus, a region implicated in feeding behavior. Increased intake of a high fat diet contributes to increased weight gain and obesity. Therefore, the current experiments investigated the effects of QRFP administration in rats and the effects of a high fat diet on prepro-QRFP mRNA and GPR103 receptor mRNA levels. Intracerebroventricular administration of QRFP-26 (3.0 nM, 5.0 nM) and QRFP-43 (1.0 nM, 3.0 nM) dose-dependently increased 1 h, 2 h, and 4 h cumulative intake of high fat (55% fat), but not low fat (10% fat) diet. In Experiment 2, hypothalamic prepro-QRFP mRNA levels and GPR103 receptor mRNA levels were measured in rats fed a high fat or a low fat diet for 21 days. Prepro-QRFP mRNA was significantly increased in the ventromedial nucleus/arcuate nucleus of the hypothalamus of rats fed a high fat diet compared to those fed a low fat diet, while GPR103 mRNA levels were unchanged. These findings suggest that QRFP is a regulator of dietary fat intake and is influenced by the intake of a high fat diet. Published by Elsevier Inc.