Initial apical migration of junctional epithelium in rats following application of lipopolysaccharide and proteases
Initial apical migration of junctional epithelium in rats following application of lipopolysaccharide and proteases
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DOI：
10.1902/jop.2005.76.1.43
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发表时间：
2005-01-01
影响因子：
4.3
通讯作者：
Watanabe, T
中科院分区：
文献类型：
--
作者：
Ekuni, D;Tomofuji, T;Watanabe, T
背景：结合上皮（JE）的顶端迁移与牙周袋的形成有关。本研究的目的是调查牙龈的变化发生在根尖迁移的JE以下的应用程序的因素与炎症性牙周病pathogenetics.Methods相关：6周龄雄性Wistar大鼠被分为6组：三个实验组来研究牙龈的变化后2，4，局部应用脂多糖（LPS）和蛋白酶8周，三个对照组使用无热原水。分别于2、4、8周后，采用末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记法（TUNEL）检测牙周膜成纤维细胞核DNA断裂，并通过增殖细胞核抗原（PCNA）表达评价基底细胞和成纤维细胞的增殖活性。结果：经LPS和蛋白酶处理的牙龈组织中PCNA阳性基底细胞增多，而成纤维细胞无明显变化。胶原蛋白的破坏，观察在2周;顶端迁移的JE和TUNEL阳性的成纤维细胞被认为是在4 weeks. Conclusions：以下应用LPS和蛋白酶大鼠龈沟，顶端迁移的JE出现同时发生的PDL成纤维细胞的凋亡，这反过来又遵循增殖的基底细胞和胶原蛋白的破坏。
Background: Apical migration of junctional epithelium (JE) occurs in association with periodontal pocket formation. The aim of this study was to investigate the gingival changes occurring during apical migration of the JE following application of factors associated with inflammatory periodontal disease pathogenesis.Methods: Six-week-old male Wistar rats were divided into six groups: three experimental groups to investigate gingival changes following 2, 4, and 8 weeks topical application of lipopolysaccharide (LPS) and proteases and three control groups using pyrogen-free water. After 2, 4 or 8 weeks, nuclear DNA fragmentation was detected in periodontal ligament (PDL) fibroblasts using the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) method, and proliferative activities of the basal cells and fibroblasts were evaluated through expression of proliferating cell nuclear antigen (PCNA). Collagen destruction was examined histologically.Results: Gingiva treated with LPS and proteases showed an increase in PCNA-positive basal cells but not the fibroblasts. Collagen destruction was observed at 2 weeks; apical migration of the JE and TUNEL-positive fibroblasts was seen at 4 weeks.Conclusions: Following application of LPS and proteases to rat gingival sulci, the apical migration of the JE appears to occur simultaneously with the apoptosis of PDL fibroblasts, which in turn follows proliferation of the basal cells and collagen destruction.