IS ESCHERICHIA COLI GROWING IN GLUCOSE-LIMITED CHEMOSTAT CULTURE ABLE TO UTILIZE OTHER SUGARS WITHOUT LAG
IS ESCHERICHIA COLI GROWING IN GLUCOSE-LIMITED CHEMOSTAT CULTURE ABLE TO UTILIZE OTHER SUGARS WITHOUT LAG
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DOI：
10.1099/00221287-141-1-71
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发表时间：
1995-01-01
期刊：
影响因子：
2.8
通讯作者：
EGLI, T
中科院分区：
文献类型：
--
作者：
LENDENMANN, U;EGLI, T
研究了在葡萄糖限制恒化器中培养的大肠杆菌是否能够与一系列糖一起生长，所述糖的利用通常在与葡萄糖的分批生长期间被抑制。在0.2、0.3和0.6 h（-1）的稀释率下生长的细胞能够立即利用果糖、甘露糖、麦芽糖和核糖并与之一起生长。半乳糖是瞬间运输的，但生长只有在相当长的滞后之后才开始。阿拉伯糖是测试的唯一糖，其既不被运输也不被立即利用。结果给出了实验证据，即在恒化培养中存在的非常低的葡萄糖浓度下，葡萄糖对体内分解代谢产物的抑制是不存在的。此外，结果表明，恒化器生长的E.大肠杆菌能够用其他几种糖代替葡萄糖作为碳源和能源，没有滞后期。
It was investigated whether Escherichia coli cultured in a glucose-limited chemostat is able to grow with a series of sugars whose utilization is normally repressed during batch growth with glucose. Cells growing at dilution rates of 0.2, 0.3 and 0.6 h(-1) were able to immediately utilize and grow with fructose, mannose, maltose and ribose. Galactose was transported instantaneously but growth started only after a considerable lag. Arabinose was the only sugar tested which was neither transported nor utilized immeadiately. The results give experimental evidence that in vivo catabolite repression by glucose is absent at the very low glucose concentrations present in chemostat culture. Additionally, the results demonstrate that chemostat-grown cells of E. coli are able to substitute glucose as a carbon and energy source by several other sugars with no lag period.