Serotonin and forskolin modulation of a chloride conductance in cultured identified Aplysia neurons.
Serotonin and forskolin modulation of a chloride conductance in cultured identified Aplysia neurons.
复制标题
血清素和毛喉素对培养的海兔神经元中氯离子电导的调节。
DOI：
10.1152/jn.1987.58.5.922
复制
发表时间：
1987
影响因子：
2.5
通讯作者：
Levitan,IB
中科院分区：
文献类型：
--
作者：
Lotshaw,DP;Levitan,IB
1. 采用电压钳技术研究了5-羟色胺（5-HT）和福斯克林对原代培养的已鉴定的泛子神经元超极化激活Cl-电导（gCl-）的影响。2. 细胞内Cl-负载诱导的超极化激活电导由Cl-携带，根据以下标准确定：外推电流的逆转电位与胆碱能Cl-电导的逆转电位非常接近，电流不受细胞外离子取代的影响，细胞外硫氰酸盐离子可逆地抑制电流，电流表现出缓慢的电压依赖指数动力学，类似于在原位的海藓神经元中描述的超极化激活的Cl-电流。3. 在鉴定的神经元B1， B2， R15和R2中，5 - ht或forskolin可逆地抑制gCl-，表明5- ht通过腺苷3‘,5’环单磷酸依赖机制起作用。4. 5 -羟色胺能抑制是由Cl通道门控电压依赖性的改变引起的。
1. The effect of serotonin (5-HT) and forskolin on a hyperpolarization activated Cl- conductance (gCl-) was studied using voltage-clamp techniques in identified Aplysia neurons maintained in primary cell culture. 2. The hyperpolarization-activated conductance induced by intracellular Cl- loading was carried by Cl- as determined by the following criteria: the extrapolated reversal potential of the current closely approximated the reversal potential of a cholinergic Cl- conductance, the current was not affected by extracellular ion substitutions other than Cl-, extracellular thiocyanate ions reversibly inhibited the current and the current exhibited slow voltage-dependent exponential kinetics similar to those described for the hyperpolarization-activated Cl- current in Aplysia neurons in situ. 3. In the identified neurons B1, B2, R15, and R2, 5-HT or forskolin reversibly inhibited gCl-, suggesting that 5-HT acted via an adenosine 3',5'-cyclic monophosphate-dependent mechanism. 4. Serotonergic inhibition resulted from a change in the voltage dependence of Cl- channel gating.