Genetic Diversity and Fungicide Sensitivity of Cytospora plurivora on Peach.
Genetic Diversity and Fungicide Sensitivity of Cytospora plurivora on Peach.
复制标题
桃多食胞孢菌的遗传多样性和杀菌剂敏感性。
DOI：
10.1094/pdis-04-22-0790-re
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发表时间：
2023
期刊：
影响因子：
4.5
通讯作者：
Kerrigan,Julia
中科院分区：
文献类型：
--
作者：
Baker,StephenT;Froelich,MarthaH;Boatwright,Harriet;Wang,Hehe;Schnabel,Guido;Kerrigan,Julia
多食细胞孢子虫D.P. Lawr., L.A. Holland & Trouillas 与南卡罗来纳州最近桃树过早衰退有关，但人们对病原体或化学控制方案知之甚少。九十三 C. 2016 年和 2017 年，分别从南卡罗来纳州 6 个城镇果园的 1 年生桃木和有症状的支架肢体中收集了多糖葡萄分离物。根据显着的 ITS1-5.8S-ITS2 序列变异性鉴定了六种独特的基因型，并将其分类为 G1 至 G6。其中三种基因型（G2、G3 和 G6）在这两年的多个地点均被高频率分离出来。除了基因型变异之外，还在基因型组之间和基因型组内观察到多种表型。使用额外的基因位点 ACT、TUB 和 EF 确定物种同一性，发现分离株属于 C。 plurivora适用于所有基因型组。所有测试的基因型对甲基托布津 (FRAC 1) 敏感，但对丙环唑和苯醚甲环唑（均为 FRAC 3）表现出稍低的敏感性。啶酰菌胺、氟吡菌酰胺（均为 FRAC 7s）、嘧菌酯和唑菌胺酯（均为 FRAC 11s）在体外对抑制念珠菌菌丝体生长无效。多食基因型。对桃树和油桃树的田间接种表明，所有基因型都产生了毒力不同的树枝溃疡病。 G1 毒性最强，G6 毒性最弱。这项研究提供了 C 之间的联系。多食基因变异性和毒力，并提供可用于改善疾病管理实践的杀菌剂敏感性信息。
Cytospora plurivoraD.P. Lawr., L.A. Holland & Trouillas has been associated with recent premature peach tree decline in South Carolina, but very little is known about the pathogen or chemical control options. Ninety-threeC. plurivoraisolates were collected in 2016 and 2017 from 1-year-old peach wood and symptomatic scaffold limbs, respectively, from orchards in six towns in South Carolina. Six unique genotypes were identified based on substantial ITS1-5.8S-ITS2 sequence variability and classified G1 to G6. Three of the genotypes (G2, G3, and G6) were isolated in high frequency in multiple locations of both years. In addition to the genotypic variation, multiple phenotypes were observed between and within genotype groups. Species identity was determined using additional gene loci: ACT, TUB, and EF, and isolates were found to belong toC. plurivorafor all genotype groups. All tested genotypes were sensitive to thiophanate-methyl (FRAC 1) but exhibited slightly lower sensitivity to propiconazole and difenoconazole (both FRAC 3). Boscalid, fluopyram (both FRAC 7s), azoxystrobin, and pyraclostrobin (both FRAC 11s) were ineffective in vitro at inhibiting mycelial growth ofC. plurivoragenotypes. Field inoculation of peach and nectarine trees revealed that all genotypes developed twig cankers with differences in virulence. G1 was most virulent, and G6 was least virulent. This study provides a link between theC. plurivoragenetic variability and virulence and provides fungicide sensitivity information that could be used to improve disease management practices.