The 5' end of two redundant sRNAs is involved in the regulation of multiple targets, including their own regulator.
The 5' end of two redundant sRNAs is involved in the regulation of multiple targets, including their own regulator.
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DOI：
10.1093/nar/gkn742
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发表时间：
2008-12
影响因子：
14.9
通讯作者：
Gottesman, Susan
中科院分区：
文献类型：
--
作者：
Guillier, Maude;Gottesman, Susan
小RNA是许多生物体中广泛存在的基因表达调控因子。这项研究描述了两个冗余的大肠杆菌sRNA，OmrA和OmrB，下调多个目标，其中大部分编码外膜蛋白的表达的作用模式。我们的研究结果表明，这两种sRNA直接与至少两种靶mRNA相互作用，ompT和cirA，在翻译起始区附近，这些目标的控制依赖于Hfq和RNase E。有趣的是，这些相互作用依赖于短的互补性，并涉及OmrA/B的保守5 ′端。该区域的突变消除了迄今为止测试的所有OmrA/B靶标的控制，从而突出了OmrA/B 5 ′端的关键作用。这使我们能够通过寻找与OmrA/B 5 ′端互补的mRNA序列，将ompR鉴定为这两种sRNA的额外直接靶点。由于OmpR转录调节因子激活OmrA和Omr B基因的表达，因此这种新鉴定的控制应导致限制OmrA/B sRNA量的自动调节环。
Small RNAs are widespread regulators of gene expression in numerous organisms. This study describes the mode of action of two redundant Escherichia coli sRNAs, OmrA and OmrB, that downregulate the expression of multiple targets, most of which encode outer membrane proteins. Our results show that both sRNAs directly interact with at least two of these target mRNAs, ompT and cirA, in the vicinity of the translation initiation region, consistent with control of these targets being dependent on both Hfq and RNase E. Interestingly, these interactions depend on short stretches of complementarity and involve the conserved 5′ end of OmrA/B. A mutation in this region abolishes control of all OmrA/B targets tested thus far, thereby highlighting the crucial role of the OmrA/B 5′ end. This allowed us, by looking for mRNA sequences complementary to the OmrA/B 5′ end, to identify ompR as an additional direct target of these two sRNAs. Since the OmpR transcriptional regulator activates expression of both omrA and omrB genes, this newly identified control should result in an autoregulatory loop limiting the amount of OmrA/B sRNAs.
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影响因子：
3.2
作者：
GRIGGS, DW;THARP, BB;KONISKY, J
通讯作者：
KONISKY, J
影响因子：
10.5
作者：
Kawamoto, H;Morita, T;Aiba, H
通讯作者：
Aiba, H
DOI：
10.1073/pnas.032066599
发表时间：
2002-04-02
影响因子：
11.1
作者：
Massé, E;Gottesman, S
通讯作者：
Gottesman, S
影响因子：
3.2
作者：
Chen, S;Zhang, AX;Storz, G
通讯作者：
Storz, G
影响因子：
3.6
作者：
Prevost, Karine;Salvail, Hubert;Masse, Eric
通讯作者：
Masse, Eric