Development and validation of an optical SPR biosensor. Assay for tylosin residues in honey
Development and validation of an optical SPR biosensor. Assay for tylosin residues in honey
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DOI：
10.1021/jf050725s
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发表时间：
2005-09-21
影响因子：
6.1
通讯作者：
Elliott, C
中科院分区：
文献类型：
--
作者：
Caldow, M;Stead, SL;Elliott, C
近年来，由于蜂房细菌性疾病对土霉素产生耐药性，泰乐素在养蜂业中的应用有所增加。基于确证质谱的方法已经开发出来，但到目前为止，还没有能够低于10 μ g kg（-1）检测限的补充筛选方法。本文介绍了一种基于光学生物传感器技术的筛选方法的开发和验证。首先将蜂蜜溶解在磷酸盐缓冲液中，然后使用Biacore Q仪器进行固相萃取（SPE）净化。采用欧盟委员会第2002/657/EC号决议中规定的定性筛选方法的标准，确定该方法的检测能力（CC β）为2.5 μ g kg（-1）。采用生物传感器筛选法和LC-MS/MS验证法对含有泰络素微量残留的蜂蜜样品进行了分析；结果很一致。
In recent years there has been an increase in the use of tylosin in apiculture as bacterial brood diseases become resistant to oxytetracycline. Confirmatory mass spectrometry based methods have been developed but up until now there has been no complementary screening method available capable of sub 10 mu g kg(-1) detection limits. In this paper the development and validation of a screening method using optical biosensor technology is presented. The honey was first dissolved in a phosphate buffer and following solid-phase extraction (SPE) cleanup was analyzed using a Biacore Q instrument. Using the criteria specified in European Commission Decision 2002/657/EC for qualitative screening methods, the detection capability (CC beta) of the method was determined to be 2.5 mu g kg(-1). Honey samples containing trace residue levels of tylosin were analyzed by both the biosensor screening method and a LC-MS/MS confirmatory procedure; the results were in good agreement.