Identification and Analysis of Potential Genes Regulated by an Alphasatellite (TYLCCNA) that Contribute to Host Resistance against Tomato Yellow Leaf Curl China Virus and Its Betasatellite (TYLCCNV/TYLCCNB) Infection in Nicotiana benthamiana
Identification and Analysis of Potential Genes Regulated by an Alphasatellite (TYLCCNA) that Contribute to Host Resistance against Tomato Yellow Leaf Curl China Virus and Its Betasatellite (TYLCCNV/TYLCCNB) Infection in Nicotiana benthamiana
复制标题
本氏烟草中由α卫星（TYLCCNA）调控的有助于宿主抵抗番茄黄化曲叶病毒及其β卫星（TYLCCNV/TYLCCNB）感染的潜在基因的鉴定和分析
DOI：
10.3390/v11050442
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发表时间：
2019-05-01
期刊：
影响因子：
4.7
通讯作者：
Xie, Yan
中科院分区：
文献类型：
--
作者：
Luo, Chaohu;Wang, Zhan Qi;Xie, Yan
最近，菜豆花叶病毒/β卫星病复合体被发现与β卫星有关，它们的存在调制了受感染植物的疾病症状和/或病毒DNA积累。然而，贝戈莫病毒/betasatellite感染过程中的生物学功能仍然不清楚。中国番茄黄化曲叶病毒（Tomato yellow leaf curl China virus，TYLCCNV）是一种广泛分布的单分型菜豆花叶病毒。在中国云南省，发现TYLCCNV/TYLCCNB疾病复合体与一颗小卫星（TYLCCNA）有关。为了阐明TYLCCNV/TYLCCNB感染和TYLCCNB/TYLCCNB/TYLCCNA引起的病毒DNA积累减少的机制，我们利用RNA测序技术分析了TYLCCNV/TYLCCNB和TYLCCNV/TYLCCNB/TYLCCNA感染的本氏烟草（Nicotiana benthamiana）幼苗的转录组。总共鉴定了2272个和1207个差异表达基因（DEG），分别响应TYLCCNV/TYLCCNB和TYLCCNV/TYLCCNB/TYLCCNA感染。与感染TYLCCNV/TYLCCNB的N.在本氏烟幼苗中，共感染TYLCCNV/TYLCCNB + TYLCCNA的植物中DEG的数量显著减少。此外，36个DEG被确定为由TYLCCNA调节，其中6个使用病毒诱导的基因沉默（VIGS）方法进行了进一步分析。沉默这6个TYLCCNA应答DEG导致更严重的疾病症状和更高的病毒DNA积累水平，表明TYLCCNA应答DEG可能减弱TYLCCNV/TYLCCNB感染。
Recently, begomovirus/betasatellite disease complexes were found to be associated with alphasatellites, and their presence modulated disease symptoms and/or viral DNA accumulation in infected plants. However, the biological functions of alphasatellites during begomovirus/betasatellite infections remain unclear. Tomato yellow leaf curl China virus (TYLCCNV) associated with a betasatellite (TYLCCNB) is a widespread monopartite begomovirus in China. In the Yunnan province of China, the TYLCCNV/TYLCCNB disease complex is found in association with an alphasatellite (TYLCCNA). In this study, in order to explain the mechanisms underlying TYLCCNV/TYLCCNB infection and reductions in viral DNA accumulation caused by TYLCCNA, we analyzed the transcriptome profiles of Nicotiana benthamiana seedlings challenged by TYLCCNV/TYLCCNB or TYLCCNV/TYLCCNB/TYLCCNA using RNA sequencing. In total, 2272 and 1207 differentially expressed genes (DEGs) were identified to respond to TYLCCNV/TYLCCNB and TYLCCNV/TYLCCNB/TYLCCNA infections, respectively. Compared with the DEGs in the TYLCCNV/TYLCCNB-infected N. benthamiana seedlings, the number of DEGs in plants co-infected with TYLCCNV/TYLCCNB + TYLCCNA was significantly reduced. Additionally, 36 DEGs were identified to be regulated by TYLCCNA, six of which were further analyzed using the virus-induced gene silencing (VIGS) approach. Silencing of these six TYLCCNA responsive DEGs caused more severe disease symptoms and higher viral DNA accumulation levels, suggesting that TYLCCNA responsive DEGs may attenuate TYLCCNV/TYLCCNB infection.