Identification and tissue distribution of odorant binding protein genes in the lucerne plant bug Adelphocoris lineolatus (Goeze)
Identification and tissue distribution of odorant binding protein genes in the lucerne plant bug Adelphocoris lineolatus (Goeze)
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紫花苜蓿 Adelphocoris lineolatus (Goeze) 气味结合蛋白基因的鉴定和组织分布
DOI：
10.1016/j.ibmb.2011.01.002
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发表时间：
2011-04-01
影响因子：
3.8
通讯作者：
Zhang, Yong-Jun
中科院分区：
文献类型：
--
作者：
Gu, Shao-Hua;Wang, Shu-Ping;Zhang, Yong-Jun
昆虫气味结合蛋白 (OBP) 是昆虫嗅觉感知所必需的，并且在将疏水性化学信息素穿过感器淋巴运输到嗅觉受体 (Ors) 方面发挥着关键作用。我们从紫花苜蓿 Adelphocoris Iineolatus (Goeze)（半翅目：Miridae）的雄性和雌性触角构建了两个高质量的 cDNA 文库，Adelphocoris Iineolatus (Goeze)（半翅目：Miridae）是一个大型且具有重要经济意义的植食性昆虫系统发育类群中的半翅目物种。总共对 1538 个男性 EST 和 1576 个女性 EST 进行了测序和分析，产生了 2915 个高质量的 EST 用于进一步分析。 2915 个 EST 被组装成 1423 个 unigene。在 1423 个 unigene 中，有 895 个 (63%) 与任何已知的 GenBank 条目没有显着相似性。 cDNA 文库中最常见的转录物是 OBP、化学感应蛋白 (CSP)、蛋白取出前体、触角特异性蛋白。我们鉴定了 14 个基因，编码 12 个“经典 OBP”，仅具有 6 个保守半胱氨酸，以及 2 个“Plus-C OBP”，具有两个额外的保守半胱氨酸和紧接在第六个半胱氨酸之后的一个保守脯氨酸。 AlinOBP4 与 LAP 具有 89% 的极高氨基酸同一性，LAP 是一种经过充分研究的变色植物盲蝽 Lygus lineolaris 的 OBP。通过实时 qPCR 测量了 14 个 OBP 在不同组织（触角、头部、胸部、腹部、腿部和翅膀）中的表达谱。结果显示，一些 OBP 基因在雄性和雌性触角中高度差异表达，其中 4 个 OBP 基因在腿中具有极高的转录水平。在两个 Plus-C OBP 基因中，一个 (AlinOBP14) 仅在头部高度表达，另一个 (AlinOBP7) 在所有嗅觉组织中表达，与其他 OBP 相比，转录水平要低得多。讨论了这些 OBP 可能的生理功能。 (C) 2011 Elsevier Ltd. 保留所有权利。
Insect odorant binding proteins (OBPs) are required for insect olfaction perception and play a key role in transporting hydrophobic semiochemicals across the sensillum lymph to the olfactory receptors (Ors). We constructed two high-quality cDNA libraries from the male and female antennae of the lucerne plant bug, Adelphocoris Iineolatus (Goeze) (Hemiptera: Miridae), a hemipteran species in a large and economically important phylogenetic group of phytophagous insects. A total of 1538 male ESTs and 1576 female ESTs were sequenced and analyzed, which produced 2915 high-quality ESTs for further analysis. The 2915 ESTs were assembled to 1423 unigenes. Of the 1423 unigenes, 895 (63%) showed no significant similarity with any known GenBank entry. The most prevalent transcripts in the cDNA libraries are OBPs, chemosensory proteins (CSPs), protein takeout precursors, antennae-specific proteins. We identified 14 genes encoding 12 "classical OBPs" with only six conserved cysteines and 2 "Plus-C OBPs" with two additional conserved cysteines and a conserved proline immediately after the sixth cysteine. AlinOBP4 has a very high amino acid identity of 89% to LAP, a well studied OBP of the tarnished plant bug Lygus lineolaris. The expression profiles of the 14 OBPs in different tissues (antennae, heads, thoraxes, abdomens, legs and wings) were measured by real-time qPCR. The results revealed some OBP genes are highly and differentially expressed in male and female antennae, and four OBP genes have an extremely high transcript level in the legs. Of two Plus-C OBP genes, one (AlinOBP14) is highly expressed only in the heads and another (AlinOBP7) is expressed in all olfactory tissues with much lower transcript levels comparing to other OBPs. The possible physiological functions of these OBPs are discussed. (C) 2011 Elsevier Ltd. All rights reserved.