New Pseudostationary Phases for Electrokinetic Chromatography: A High‐Molecular Surfactant and Proteins
New Pseudostationary Phases for Electrokinetic Chromatography: A High‐Molecular Surfactant and Proteins
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电动色谱的新准固定相：高分子表面活性剂和蛋白质
DOI：
10.1002/jccs.199400036
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发表时间：
1994
影响因子：
1.8
通讯作者：
Yoshihide Tanaka
中科院分区：
文献类型：
--
作者：
S. Terabe;Hiroto Ozaki;Yoshihide Tanaka
为了扩大电动色谱（EKC）的适用性，介绍了两种新型的假固定相。采用高分子表面活性剂丙烯酸丁酯/甲基丙烯酸丁酯/甲基丙烯酸共聚物（BBMA）作为胶束形成表面活性剂用于微束电动色谱（MEKC）。BBMA的临界胶束浓度基本为零，这意味着胶束浓度是恒定的，与温度和缓冲液无关。研究了BBMA作为MEKC假固定相的一些特性，并与传统离子表面活性剂进行了比较。卵泡样蛋白和亲和蛋白是亲和型EKC中有用的手性选择物。对映异构体与这些蛋白质分离的例子如下。
To extend the applicability of electrokinetic chromatography (EKC), two new types of pseudostationary phases have been introduced. A high-molecular surfactant, butyl acrylate/butyl methacrylate/methacrylic acid copolymer (BBMA) is employed as a micellar forming surfactant for miccllar electrokinetic chromatography (MEKC). The critical micelle concentration of BBMA is essentially zero, which means the micellar concentration is constant irrespective of temperature and buffer. Some characteristic features of BBMA as the pseudostationary phase for MEKC is investigated in comparison with conventional ionic surfactants. Ovomucoid and avidin, which are proteins isolated from egg white, have been found to be useful chiral selectors in affinity EKC. A few examples of the separation of enantiomers with these proteins are shown.