Detection of a protein-acetaldehyde adduct in the liver of rats fed alcohol chronically.
Detection of a protein-acetaldehyde adduct in the liver of rats fed alcohol chronically.
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长期饮酒大鼠肝脏中蛋白质-乙醛加合物的检测。
DOI：
10.1172/jci113362
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Lumeng,L
中科院分区：
文献类型：
--
作者：
Lin,RC;Smith,RS;Lumeng,L
我们在此报告了当大鼠长期饮酒时，肝脏中蛋白质-乙醛加合物（蛋白质-AA）的体内形成。这种化学修饰的蛋白质通过电免疫印迹技术和识别乙醛加合物作为表位的兔多克隆抗体（即抗血蓝蛋白-AA IgG 和抗肌红蛋白-AA IgG）得到证实。它的分子量为37,000。在仅喂食美国营养研究所 1976 年含酒精流质饮食 1 周的大鼠的肝脏中可以检测到该物质。由于酒精喂养大鼠和对照大鼠的可溶性肝蛋白的蛋白质谱在 SDS-PAGE 上是相同的，因此电免疫印迹显示的过氧化物酶阳性条带很可能不是从头合成的新蛋白质。不需要硼氢化物还原来稳定该蛋白质-AA。在 24 小时内，以 8 小时的间隔向大鼠腹腔注射乙醇（2 g/kg 体重），未在肝脏中产生任何可检测到的蛋白质-AA。将对照肝脏的肝脏匀浆与不含氰基硼氢化钠的乙醛一起孵育 4 小时也未能产生任何蛋白质-AA。因此，本文报道的体内 37 kD 蛋白质 AA 的形成取决于长期饮酒。
We report here the formation in vivo of a protein-acetaldehyde adduct (protein-AA) in liver when rats were fed alcohol chronically. This chemically modified protein was demonstrated by electroimmunotransblot technique and with rabbit polyclonal antibodies that recognize acetaldehyde adduct as an epitope (i.e., both anti-hemocyanin-AA IgG and anti-myoglobin-AA IgG). It has a molecular weight of 37,000. It can be detected in the liver of rats fed the alcohol-containing American Institute of Nutrition 1976 liquid diet for only 1 wk. Since the protein profiles of soluble hepatic proteins from alcohol-fed and control rats were identical on SDS-PAGE, the peroxidase-positive band demonstrated by electroimmunotransblot was most likely not a new protein synthesized de novo. Borohydride reduction was not necessary to stabilize this protein-AA. Intraperitoneal injections of ethanol (2 g/kg body wt) at 8-h intervals to rats over a 24-h period did not produce any detectable protein-AA in the liver. Incubation of the liver homogenate from a control liver with acetaldehyde without sodium cyanoborohydride for 4 h also failed to generate any protein-AA. Therefore, the formation of the 37-kD protein-AA in vivo reported here is dependent on chronic alcohol consumption.Images