Preincubation with a low concentration of methyl-beta-cyclodextrin enhances baculovirus expression system productivity
Preincubation with a low concentration of methyl-beta-cyclodextrin enhances baculovirus expression system productivity
复制标题
低浓度甲基-β-环糊精预孵育可提高杆状病毒表达系统的生产力
DOI：
10.1007/s10529-019-02708-z
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发表时间：
2019
影响因子：
2.7
通讯作者：
Hao Bifang
中科院分区：
文献类型：
--
作者：
Huang Jinshan;Liu Na;Shen Xingjia;Hao Bifang
目的利用杆状病毒表达系统提高外源蛋白在培养细胞中的产量。结果低浓度的MβCD，最佳应用浓度为0.2 5 mM，适宜的预孵育时间为10 ~ 12 0 min，结论用低浓度MβCD预孵育可提高杆状病毒感染和外源蛋白的表达水平表达生产力。
ObjectivesTo enhance the productivity of foreign protein in culture cells using baculovirus expression system.ResultsA low concentration of MβCD, with the optimal application concentration of 0.25 mM and the appropriate preincubation time range from 10 to 120 min, can efficiently enhance expression levels in both the AcMNPV and BmNPV expression systems.ConclusionsPreincubation with a low concentration MβCD enhance baculovirus infection and foreign protein expression productivity.