Dietary SWF (R) enhanced growth performance and disease resistance in hybrid sturgeon (Acipenser baerii x Acipenser schrenckii) mediated by the gut microbiota
Dietary SWF (R) enhanced growth performance and disease resistance in hybrid sturgeon (Acipenser baerii x Acipenser schrenckii) mediated by the gut microbiota
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膳食 SWF (R) 通过肠道微生物群介导增强杂交鲟（Acipenser baerii x Acipenser schrenckii）的生长性能和抗病能力
DOI：
10.1016/j.aqrep.2020.100346
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发表时间：
2020
影响因子：
3.7
通讯作者：
Zhang Zhen
中科院分区：
文献类型：
--
作者：
Teame Tsegay;Wu Xuexiang;Hao Qiang;Ding Qianwen;Liu Hongliang;Ran Chao;Yang Yalin;Xia Liqiu;Wei Shaojun;Zhou Zhigang;Zhang Youming;Zhang Zhen
在鱼类的胃肠道中存在健康的肠道微生物群对于肠道的最佳功能是重要的，肠道微生物群对宿主的生长和健康起着重要的作用。本研究旨在探讨饲料应激无忧(SWF®)对施氏鲟鱼(Acipenser Baerii X Acipenser Schrenckii)生长、饲料利用和抗病能力的影响。用SWF®添加饲料或基础饲料饲养鲟鱼3周。添加SWF®显著提高了鲟鱼的增重和饲料系数(P<；0.05)。饲料中添加SWF®可提高鲟鱼对A的抵抗力。VeroniiHm091(P=0.09)。在肠道微生物区系方面，与对照组相比，SWF®组肠道细菌总数、梭杆菌、纤毛虫和变形杆菌显著增加(P<；0.05)，而放线菌显著减少(P<；0.01)。此外，在实验结束时，鲟鱼的肠道微生物区系被定植到无菌斑马鱼(GF)上，为期三天。结果表明，生长促进基因mTOR、MyoD和Mygenin在被SWF®组鲟鱼肠道微生物群定植的GF斑马鱼中的表达显著增强。此外，经肠道微生物区系定植的GF斑马鱼的转化生长因子-β显著增加(P<；0.01)，而肿瘤坏死因子-α的表达显著降低(P<；0.05)。非特异性免疫相关基因DEFBL-1、C3a和溶菌酶的表达显著增加(P<；0.05)。用摄食SWF®的鲟鱼肠道微生物群定植的GF斑马鱼对A的存活率显著高于对照组。VeroniiHm091(P<；0.05)。我们的研究表明，SWF®诱导的肠道微生物区系对利用GF斑马鱼模型鲟鱼的生长和抗病起到了重要作用。
The presence of healthy gut microbiota in the gastrointestinal tract of fish is important for the optimal function of gut, which plays a significant role in the host growth and health. The aim of the present study was to investigate the effect of dietary stress worry free (SWF®) on growth, feed utilization and disease resistance of hybrid sturgeon (Acipenser baerii x Acipenser schrenckii). Sturgeon were fed for three weeks with SWF® supplemented or basal diet. The weight gain and FCR of sturgeon fed on the diet supplemented with SWF® were significantly improved (P<0.05). SWF® supplemented diet provoked an increase in the resistance of sturgeon againstA. veroniiHm091 (P=0.09). In terms of gut microbiota, the number of total bacteria,Fusobacteria, Firmicutes,andProteobacteriawere increased significantly in the SWF® group (P<0.05), whereas significant reduction ofActinobacteriawas observed in the gut of the SWF® group compared with the control group (P<0.01). Moreover, at the end of the experiment the gut microbiota of sturgeon, were colonized to germ-free (GF) zebrafish for three days. Results indicated that, the expression of growth promoter genes mTOR, MyoD and Myogenin was significantly higher in GF zebrafish colonized with gut microbiota of SWF® group of sturgeon. Furthermore, TGF-β was increased significantly in GF zebrafish colonized with gut microbiota from SWF® group (P<0.01), whereas the expression of TNF-α was significantly decreased (P<0.05). The expression of non-specific immune related genes DEFBL-1, C3a and Lysozyme was significantly increased in GF zebrafish colonized with gut microbiota of sturgeon fed on SWF® (P<0.05). Group of GF zebrafish colonized with gut microbiota of sturgeon fed on SWF® had significantly higher survival rate againstA. veroniiHm091 (P<0.05). Our study suggests that, the gut microbiota induced by SWF® played a great role in growth and disease resistance of sturgeon using GF zebrafish model.