Pyrethroids and piperonyl-butoxide affect human T-lymphocytes in vitro
Pyrethroids and piperonyl-butoxide affect human T-lymphocytes in vitro
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DOI：
10.1016/s0378-4274(99)00032-6
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发表时间：
1999-06-30
影响因子：
3.5
通讯作者：
Diel, E
中科院分区：
文献类型：
--
作者：
Diel, F;Horr, B;Diel, E
合成拟除虫菊酯越来越多地用作杀虫剂，据称对人体的毒性相对较低。本研究的目的是检查合成拟除虫菊酯S-生物丙烯菊酯单独使用以及与常见的胡椒基丁醚（PBO）联合使用对特应性个体和非特应性对照受试者的人血液淋巴细胞和嗜碱性粒细胞的体外影响。S-生物丙烯菊酯和PBO也引起抑制淋巴细胞增殖（MTT试验）后，72小时的培养期，在浓度依赖性的方式。与MTT测量相反，联合药物在抑制白细胞介素-4（IL-4）和干扰素-γ（IFN-γ）产生方面更有效。在微摩尔S-生物丙烯菊酯存在下培养24-48 h后，特应性和非特应性受试者之间的调节性IL-4/IFN-γ平衡显示出显著差异（P < 0.001）。此外，S-生物丙烯菊酯，PBO和联合药物诱导组胺释放的人嗜碱性粒细胞。虽然这种作用与组胺释放剂如FMLP和抗IgE相比很小，但与非特异性供者相比，特应性供者对S-生物丙烯菊酯和PBO的反应有显著差异（P < 0.01）。在划痕试验实验中，18名测试的特应性志愿者中有4名对S-生物丙烯菊酯和二氯苯醚菊酯表现出阳性反应（风团和耀斑），而对照组（年龄匹配）中未检测到反应。这些发现证明了合成拟除虫菊酯S-生物丙烯菊酯与协同PBO联合使用人淋巴细胞和嗜碱性粒细胞的这种体外方法的免疫和过敏毒理学特性。(C)1999爱思唯尔科学爱尔兰有限公司保留所有权利。
Synthetic pyrethroids are increasingly used as insecticides and are claimed to have a relatively low human toxicity. The aim of this study was to examine the in vitro effects of the synthetic pyrethroid S-bioallethrin alone and in combination with the common synergist piperonyl-butoxide (PBO) on human blood lymphocytes and basophils in atopic individuals and non-atopic control subjects. S-bioallethrin and PBO also caused inhibition of lymphocyte proliferation (MTT-test) after a 72-h culture period in a concentration dependent manner. In contrast to the MTT-measurements the combined agents are more effective in inhibiting interleukin-4 (IL-4)- and interferon-gamma (IFN-gamma)-production. The regulatory IL-4/IFN-gamma balance showed a significant difference between atopic and nonatopic subjects after a culture period of 24-48 h in the presence of micromolar S-bioallethrin (P < 0.001). Furthermore S-bioallethrin, PBO and the combined agents induced histamine release from human basophils. Although this effect was little compared to histamine liberators like FMLP and anti-IgE, the response to S-bioallethrin and PBO was significantly different in atopic donors compared with non-atopics (P < 0.01). In scratch test experiments 4 of 18 tested atopic volunteers showed positive reaction (wheals and flares) to S-bioallethrin and permethrin, whereas no reaction could be measured in the control group (age-matched). These findings demonstrate the immuno- and allergo-toxicological properties of the synthetic pyrethroid S-bioallethrin combined with the synergistic PBO using this in vitro approach with human lymphocytes and basophils. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.