P-fimbriated clones among uropathogenic Escherichia coli strains
P-fimbriated clones among uropathogenic Escherichia coli strains
复制标题
尿路致病性大肠杆菌菌株中的 P 菌毛克隆
DOI：
10.1128/iai.43.1.149-155.1984
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发表时间：
1984
影响因子：
3.1
通讯作者：
Timo K. Korhonen
中科院分区：
文献类型：
--
作者：
V. Väisänen;J. Elo;E. Väisänen;A. Siitonen;I. Orskov;F. Orskov;S. Svenson;P. Mäkelä;Timo K. Korhonen
对237株从各种泌尿系感染患者尿液和健康儿童粪便中分离的大肠埃希菌进行了O群、K1荚膜、1型菌毛、P菌毛、X粘附素和溶血素的检测。还分析了一些菌株的K和H抗原、外膜蛋白模式和质粒含量。P菌毛，溶血素生产，和某些O组被发现与肾盂肾炎显着相关。拥有1型菌毛或K1胶囊或质粒含量与毒力没有显着相关性。分析了139株较常见的O群菌株的外膜蛋白图谱。只有一到三个模式，不同血清型之间，通常发现在任何一个O组。独特的群体（克隆）被发现时，根据完整的血清型，菌毛，溶血素生产，外膜蛋白模式的菌株进行分组;此外，质粒的平均数是典型的菌株在一个给定的克隆。7个克隆与肾盂肾炎被发现，他们一起占57%的O血清型菌株的肾盂肾炎患者。这7个克隆为P菌毛型，但它们的血清型不同如下：O1：K1：H7、O4：K12：H1、O4：K12：H5、O6：K2：H1、O16：K1：H6或O18ac：K5：H7。所有O1：K1：H7菌株根据1型菌毛和溶血素产生的存在或不存在分为两个克隆。还发现了一个与膀胱炎相关的克隆;这包括缺乏P菌毛的O 6：K13：H1菌株。在健康儿童的粪便菌株中没有发现这8个克隆的任何代表。它们被认为是具有引起人类尿路感染特殊能力的毒性克隆。
A total of 237 Escherichia coli strains isolated from the urine of patients with various forms of urinary tract infection or from feces of healthy children were analyzed for O group, possession of K1 capsule, type 1 fimbriae, P fimbriae, X adhesin, and production of hemolysin. Some of the strains were also analyzed for K and H antigens, outer membrane protein pattern, and plasmid content. P fimbriation, hemolysin production, and certain O groups were found to be significantly correlated with pyelonephritogenicity. Possession of type 1 fimbriae or of K1 capsule or plasmid content did not significantly correlate with virulence. Outer membrane protein patterns in 139 strains of the more common O groups were analyzed. Only one to three patterns, which varied between serotypes, were usually found within any one O group. Distinctive groups (clones) were found when the strains were grouped according to complete serotype, fimbriation, hemolysin production, and outer membrane protein pattern; also, the mean number of plasmids was typical of the strains in a given clone. Seven clones associated with pyelonephritis were found; together they accounted for 57% of the O serotypable strains from the pyelonephritis patients. The seven clones were P fimbriated but differed in their serotypes as follows: O1:K1:H7, O4:K12:H1, O4:K12:H5, O6:K2:H1, O16:K1:H6, or O18ac:K5:H7. All O1:K1:H7 strains observed fell into two clones according to the presence or absence of type 1 fimbriae and hemolysin production. One clone associated with cystitis was also found; this consisted of O6:K13:H1 strains lacking P fimbriae. Not a single representative of these eight clones was found among the fecal strains from the healthy children. They are proposed to represent virulent clones with special ability to cause human urinary tract infection.