Flavobacterium laiguense sp. nov., a psychrophilic bacterium isolated from Laigu glacier on the Tibetan Plateau
Flavobacterium laiguense sp. nov., a psychrophilic bacterium isolated from Laigu glacier on the Tibetan Plateau
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莱古黄杆菌 sp.
DOI：
10.1099/ijsem.0.003400
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发表时间：
2019
影响因子：
2.8
通讯作者：
Xin Yuhua
中科院分区：
文献类型：
--
作者：
Yang Leilei;Liu Qing;Liu Hongcan;Zhou Yuguang;Xin Yuhua
从青藏高原的来古冰川中分离到一株细菌，命名为LB 2 P30 T。菌株LB 2 P30 T是一株需氧、非运动、杆状、黄色、革兰氏染色阴性的细菌。生长的温度范围为4-20 °C（最佳，14 °C）。基于16 S rRNA基因部分序列的系统发育分析表明，菌株LB 2 P30 T属于黄杆菌属，与Flavobacterium fluvii（98.12%）和Flavobacterium limicola（97.91%）的同源性最高。 LB 2 P30 T与F. FluviiDSM 19978TandF. LimicolaDSM 15094 T的转化率分别为76.73%和77.40%。 菌株LB 2 P30 T的细胞含有作为主要脂肪酸的总特征3（包括C16：1ω 7 c和/或C16：1ω6c）和异-C15：0以及作为唯一甲基萘醌的甲基萘醌-6。 基因组DNA G+C含量为34.74mol%。基于表型、化学分类学、基因型和系统发育特征，我们将菌株LB 2 P30 T命名为Flavobacterium laiguensesp.模式菌株为LB 2 P30 T（= CGMCC1.11271T =NBRC 113059 T）。
One bacterial strain, denoted as LB2P30T, was isolated from Laigu glacier located on the Tibetan Plateau, PR China. Strain LB2P30Twas an aerobic, non-motile, rod-shaped, yellow-pigmented and Gram-stain-negative bacterium. The temperature range for growth was 4–20 °C (optimum, 14 °C). The phylogenetic analysis based on partial 16S rRNA gene sequences showed that strain LB2P30Tbelonged to the genusFlavobacteriumand was most similar toFlavobacterium fluvii(98.12 %) andFlavobacterium limicola(97.91 %). The average nucleotide identity values between strain LB2P30Tand its closest relatives,F. fluviiDSM 19978TandF. limicolaDSM 15094T, were 76.73 % and 77.40 %, respectively. Cells of strain LB2P30Tcontained summed feature 3 (comprising C16:1ω7cand/or C16:1ω6c) and iso-C15 : 0as the predominant fatty acids and menaquinone-6 as the sole menaquinone. The genomic DNA G+C content was 34.74 mol%. Based on the phenotypic, chemotaxonomic, genotypic and phylogenetic characteristics, we propose strain LB2P30Tas a novel species of the genusFlavobacteriumwith the nomenclature ofFlavobacterium laiguensesp. nov. The type strain is LB2P30T(=CGMCC 1.11271T=NBRC 113059T).