The role of phosphoinositide 3-kinase in adhesion of oral epithelial cells to titanium
The role of phosphoinositide 3-kinase in adhesion of oral epithelial cells to titanium
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DOI：
10.1016/j.archoralbio.2013.07.013
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发表时间：
2013-11-01
影响因子：
3
通讯作者：
Koyano, Kiyoshi
中科院分区：
文献类型：
--
作者：
Atsuta, Ikiru;Ayukawa, Yasunori;Koyano, Kiyoshi
背景资料：口腔上皮细胞（Oral epithelial cells，OECs）在钛表面的粘附可以提高种植体修复的成功率。目的：研究口腔上皮细胞在钛种植体表面的粘附机制。材料和方法：（1）在存在或不存在磷酸肌醇3-激酶（PI 3 K）激活剂或抑制剂和/或生长因子的情况下，在钛板（Ti）或培养皿上培养大鼠OEC后，并对这些条件下的OEC形态进行分析。(2)拔除右侧上颌第一磨牙，并替换为实验种植体。结果：（1）OECs在钛板上的贴壁能力、整合素β 4水平和F-actin结构的连续性均低于钛板。在PI 3 K抑制后，显著降低对两种底物的粘附。相比之下，用激活剂或胰岛素样生长因子激活PI 3 K将OEC粘附和Ti上粘附分子的表达恢复到在培养皿上培养的OEC中观察到的水平。细胞迁移受PI 3 K激活的抑制。(2)结论：PI 3 K在OECs与Ti的粘附过程中起重要作用。(C)2013爱思唯尔有限公司保留所有权利。
Background: Oral epithelial cells (OECs) adhesion to titanium may improve the success rate of implant restoration.Purpose: We investigated the mechanism by which OECs adhere to titanium dental implants.Materials and methods: (1) After culturing rat OECs on titanium plates (Ti) or culture dishes in the presence or absence of a phosphoinositide 3-kinase (PI3K) activator or inhibitors and/or growth factors, and OEC morphology under these conditions were analyzed. (2) Right maxillary first molars were extracted and replaced with experimental implants. The rats were treated with or without growth factors.Results: (1) Cell adherence was lower of OECs on Ti than in those on culture dishes, as were the levels of integrin beta 4 and the continuity of F-actin structures. After PI3K inhibition, markedly reducing adherence to both substrates. In contrast, PI3K activation with activator or insulin-like growth factor restored the OEC adherence and the expression of adhesion molecules on Ti to the levels seen in OECs cultured on dishes. Cell migration was inhibited by PI3K activation. (2) High expression of integrin beta 4 was observed in the peri-implant epithelia of PI3K-activated rats.Conclusion: These findings suggest that PI3K plays an important role in the adhesion of OECs to Ti. (C) 2013 Elsevier Ltd. All rights reserved.