NEUTROPHIL-MEDIATED POST-ISCHEMIC TUBULAR LEAKAGE IN THE RAT-KIDNEY
NEUTROPHIL-MEDIATED POST-ISCHEMIC TUBULAR LEAKAGE IN THE RAT-KIDNEY
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DOI：
10.1038/ki.1989.230
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发表时间：
1989-10-01
影响因子：
19.6
通讯作者：
KALLSKOG, TOK
中科院分区：
文献类型：
--
作者：
HELLBERG, POA;KALLSKOG, TOK
用抗中性粒细胞血清（ANS）诱导雄性sd大鼠中性粒细胞减少。对照组给予等量的无活性血清。单侧完全性肾缺血45分钟后，用电磁流量计测定肾血流量（RBF）。采用微穿刺技术测定肾小球毛细血管的净滤过力（NFF）、单肾元滤过率（SNGFR）和小管阻塞频率。在正常对侧肾缺血后近端和远端小管表面注射3H-或14c -胰岛素，测量小管渗漏的分数恢复。ANS和无活性血清对正常肾脏的菊粉清除率（CIn）均无影响。在缺血后的肾脏中，ans处理的CIn比对照动物高4倍。中性粒细胞耗损动物和对照动物的RBF、NFF、SNGFR和小管阻塞频率无差异。注射菊粉至近端小管的菊粉经小管渗漏在ans治疗组明显少于对照组(11.3。1.5% vs. 35.1。6.5%;P < 0.01)。但两组远端肾小管渗漏相等。对照组表现为等血尿(350 .+-。29 . most .cntdot。而接受ans治疗的动物产生高渗尿（555 .+- 1）。60 mOsm .cntdot。公斤;P < 0.05)。由此可见，中性粒细胞介导缺血后肾小管渗漏，导致肾清除率降低，不能产生高渗尿。
Neutropenia was induced in male Sprague-Dawley rats by administration of antineutrophil serum (ANS). A control group received an equal volume of inactive serum. After 45 minutes of unilateral complete renal ischemia the renal blood flow (RBF) was measured by an electromagnetic flow meter. The net filtration force (NFF) in glomerular capillaries, single nephron filtration rate (SNGFR) and frequency of tubular obstructions were estimated by a micropuncture technique. Tubular leakage was measured from the fractional recovery in the normal contralateral kidney of 3H- or 14C-insulin injected into surface proximal and distal tubules of the post-ischemic kidney. Neither ANS nor inactive serum had any influence on inulin clearance (CIn) in the normal kidney. In the post-ischemic kidney, CIn was four times higher in ANS-treated than in control animals. There was no difference in RBF, NFF, SNGFR or the frequency of tubular obstructions between neutrophil-depleted and control animals. The transtubular leakage of inulin injected into proximal tubules was substantially less in the ANS-treated than in the control group (11.3 .+-. 1.5% vs. 35.1 .+-. 6.5%; P < 0.01). But distal tubular leakage was equal in the two groups. The control group showed isosthenuria (350 .+-. 29 mOsm .cntdot. kg-1), while ANS-treated animals produced hyperosomolar urine (555 .+-. 60 mOsm .cntdot. kg-1; P < 0.05). It is concluded that neutrophil granulocytes mediate post-ischemic tubular leakage, which contributes to the depression in renal clearance parameters and the inability to produce hyperosomolar urine.