Evaluation of a root extract gel from Urtica dioica (Urticaceae) as analgesic and anti-inflammatory therapy in rheumatoid arthritis in mice
Evaluation of a root extract gel from Urtica dioica (Urticaceae) as analgesic and anti-inflammatory therapy in rheumatoid arthritis in mice
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DOI：
10.4314/tjpr.v15i4.16
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发表时间：
2016-04-01
影响因子：
0.6
通讯作者：
Cai, Dao-Zhang
中科院分区：
文献类型：
--
作者：
Liao, Jia-Cheng;Wei, Zhao-Xia;Cai, Dao-Zhang
目的：研制一种治疗小鼠关节炎的止痛中药凝胶剂，并对其进行表征。方法：采用Carbopol 934为原料，制备了一种用于治疗小鼠关节炎的止痛凝胶。以2%吲哚美辛凝胶为标准，对制备的凝胶进行了颜色、外观、pH、质地、粘度等各项物理测试和对小鼠的皮肤刺激性、止痛性、抗炎等体内评价。其pH值为7.1，粘度为21.2cps。凝胶呈现假塑性流变性，表现为剪切变稀，剪切速率增大。在小鼠皮肤刺激性试验中对皮肤无刺激性，在角叉菜胶诱导的大鼠足爪肿胀模型中，24 h后对足肿胀的抑制率为55.05%，与标准凝胶的53.93%相当。扭体试验表明，该凝胶的止痛率为58.21%，而吲哚美辛标准凝胶的止痛率为61.19%。结论：虎杖甲醇根提取物外用凝胶可能是治疗类风湿关节炎的一种有效、安全的替代非类固醇消炎药的药物，但其安全性和临床疗效有待进一步研究。
Purpose: To develop and characterize an herbal gel prepared from methanol root extract of Urtica dioica (Urticaceae) (Stinging nettle) for the treatment of arthritis in mice.Methods: A methanol root extract from Urtica dioica was prepared, and a gel was then prepared using Carbopol 934. The prepared gel was subjected to various physical tests (color, appearance, pH, texture, viscosity) and in vivo evaluation, including primary skin irritation, analgesic, and anti-inflammatory tests, in arthritic mice and compared with 2 % indomethacin gel, which was used as standard.Results: The prepared herbal gel was of light gray color with a smooth texture. It showed a pH of 7.1 and a viscosity of 21.2 cps. The gel exhibited pseudoplastic rheology, as evidenced by shear thinning with increased shear rate. It was non-irritating to the skin in primary skin irritation test in mice and showed 55.05 % inhibition of paw edema in a carrageenan-induced hind rat paw edema model, comparable to that of the standard gel (53.93 %), after 24 h. The gel showed 58.21 % analgesia, versus 61.19 % analgesia for the indomethacin gel standard in writhing test.Conclusion: The topical gel from methanol root extract of U. dioica may be an efficacious and safe alternative to non-steroidal anti-inflammatory drugs in the treatment of rheumatoid arthritis but this requires further investigations to ascertain its safety and clinical efficacy.