OnLine LC-UV-ESI-MS/MS Comparative Analysis of Changes in Goat Colostrum N/O-Glycopatterns at Different Parities.
OnLine LC-UV-ESI-MS/MS Comparative Analysis of Changes in Goat Colostrum N/O-Glycopatterns at Different Parities.
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DOI：
10.1021/acs.jafc.9b07075
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发表时间：
2020-01
影响因子：
6.1
通讯作者：
Zhongfu Wang;Yu Lu;W. Jin;Yuerong Yang;Yueqing Jia;Lujia Sun;Jie Liu;Langhong Wang;Fuxin Zhang;Wupeng Ge;Jiansheng Wang;Linjuan Huang
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文献类型：
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作者：
Zhongfu Wang;Yu Lu;W. Jin;Yuerong Yang;Yueqing Jia;Lujia Sun;Jie Liu;Langhong Wang;Fuxin Zhang;Wupeng Ge;Jiansheng Wang;Linjuan Huang
羊奶低聚糖是一种具有多种生物学功能的复杂碳水化合物。山羊乳中的游离低聚糖比牛乳中的游离低聚糖更接近于人乳。目前，对不同胎次山羊乳糖复合物的变化研究较少。在此，我们定性和定量比较了山羊乳糖蛋白N/O-糖组在不同的奇偶性，使用稳定的同位素标记，然后电喷雾电离质谱和在线亲水相互作用色谱。N-聚糖主要为岩藻糖基化和非岩藻糖基化非唾液酸化，岩藻糖基化和唾液酸化均随着产次的增加而逐渐增加，（在第三次产次时）分别为第一次产次的1.25倍和3.3倍。O-聚糖大部分为非岩藻糖基化非唾液酸化，唾液酸化随产次增加而增加，第三次产次的Neu 5Ac-唾液酸化比第一次产次高9倍，而Neu 5GC-唾液酸化高5.5倍。本研究为探索最接近母乳的替代奶源，开发人性化配方奶提供了参考。
Goat milk oligosaccharides are complex carbohydrates with a variety of biological functions. Free oligosaccharides from goat milk show more similarity to human than cow milk. At present, changes in goat milk glycoconjugates at different parity remain poorly studied. Herein, we qualitatively and quantitatively compared the goat milk glycoprotein N/O-glycome at different parity using stable isotope labeling followed by electrospray ionization mass spectrometry and online hydrophilic interaction chromatography. N-glycans were mainly fucosylated and non-fucosylated non-sialylated, and both fucosylation and sialylation gradually increased with parity, amounting (at the third parity) to 1.25 times and 3.3 times those of the first parity, respectively. O-glycans were mostly non-fucosylated non-sialylated, and sialylation increased with increasing parity, and Neu5Ac-sialylated was up to 9 times higher in the third parity than in the first parity, whereas Neu5Gc-sialylated was 5.5 times higher. This study provides a reference for exploring an alternative milk source closest to human milk, and for the development of humanized formula milk.