RELATIONSHIP BETWEEN GOLGI APPARATUS, GERL, AND SECRETORY GRANULES IN ACINAR CELLS OF RAT EXORBITAL LACRIMAL GLAND
RELATIONSHIP BETWEEN GOLGI APPARATUS, GERL, AND SECRETORY GRANULES IN ACINAR CELLS OF RAT EXORBITAL LACRIMAL GLAND
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DOI：
10.1083/jcb.74.2.399
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发表时间：
1977-01-01
影响因子：
7.8
通讯作者：
OLIVER, C
中科院分区：
文献类型：
--
作者：
HAND, AR;OLIVER, C
应用电镜形态学和细胞化学技术，研究了大鼠眶外泪腺腺泡细胞分泌颗粒的形成方法。高尔基体内面的未成熟分泌颗粒经常附着在一个狭窄的脑池结构上，类似于神经元中描述的GERL [高尔基内质网溶酶体]。在泪腺中，GERL位于邻近内高尔基体球囊，或以不同的距离与其分开。部分GERL常被修饰的粗面内质网池（RER）紧密包围，其邻近GERL的表面缺乏核糖体。分泌酶过氧化物酶的二氨基联苯胺反应产物定位于核膜的池，RER，外周高尔基囊泡，高尔基球囊，和未成熟和成熟的分泌颗粒。GERL通常不含过氧化物酶反应产物或仅含少量。硫胺素焦磷酸酶反应产物存在于2-4个内高尔基体球囊中;偶尔，最内侧球囊扩张并有孔，并且比相邻球囊含有较少的反应产物。酸性磷酸酶反应产物存在于GERL，不成熟的颗粒，很少，在最里面的球囊，但不是在其余的高尔基体球囊。在100 kV电压下观察的Acceptor制备的厚切片显示，GERL由脑池和有孔或管状部分组成。未成熟颗粒通过与管状部分的多个连接而附着于GERL。在大鼠眶外泪腺中，高尔基体囊泡可能参与分泌蛋白的转运，GERL可能参与分泌颗粒的形成。
The method of secretory granule formation in the acinar cells of the rat exorbital lacrimal gland was studied by EM morphological and cytochemical techniques. Immature secretory granules at the inner face of the Golgi apparatus were frequently attached to a narrow cisternal structure similar to GERL [Golgi endoplasmic reticulum lysosomes] as described in neurons. In the lacrimal gland, GERL was located adjacent to the inner Golgi saccule, or separated from it by a variable distance. Portions of GERL were often closely paralleled by modified cisternae of rough endoplasmic reticulum (RER), which lacked ribosomes on the surface adjacent to GERL. Diaminobenzidine reaction product of the secretory enzyme peroxidase was localized in the cisternae of the nuclear envelope, RER, peripheral Golgi vesicles, Golgi saccules, and immature and mature secretory granules. GERL was usually free of peroxidase reaction product or contained only a small amount. Thiamine pyrophosphatase reaction product was present in 2-4 inner Golgi saccules; occasionally, the innermost saccule was dilated and fenestrated and contained less reaction product than the next adjacent saccule. Acid phosphatase (AcPase) reaction product was present in GERL, immature granules, and, rarely, in the innermost saccule, but not in the rest of the Golgi saccules. Thick sections of AcPase preparations viewed at 100 kV revealed that GERL consisted of cisternal, and fenestrated or tubular portions. The immature granules were attached to GERL by multiple connections to the tubular portions. In the rat exorbital lacrimal gland, the Golgi saccules may participate in the transport of secretory proteins and GERL is probably involved in the formation of secretory granules.