CONTROL OF FOLLICULAR EPITHELIUM DEVELOPMENT AND VITELLINE ENVELOPE FORMATION IN THE MOSQUITO - ROLE OF JUVENILE-HORMONE AND 20-HYDROXYECDYSONE
CONTROL OF FOLLICULAR EPITHELIUM DEVELOPMENT AND VITELLINE ENVELOPE FORMATION IN THE MOSQUITO - ROLE OF JUVENILE-HORMONE AND 20-HYDROXYECDYSONE
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DOI：
10.1016/0040-8166(91)90015-l
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发表时间：
1991-01-01
期刊：
影响因子：
2.6
通讯作者：
LEA, AO
中科院分区：
文献类型：
--
作者：
RAIKHEL, AS;LEA, AO
应用显微外科手术、激素应用和透射电子显微镜技术，研究了埃及伊蚊卵巢初级卵泡中卵泡上皮分化和卵黄膜形成的调控。羽化后前3天，初级卵泡生长，卵泡上皮细胞分化，线粒体、粗面内质网和高尔基复合体含量显著增加。卵泡上皮的生长和分化似乎受保幼激素(JH)的控制，因为它们被新近关闭的成年雌性的翼体切除所阻止，并可以通过植入翼体或应用JH III来恢复。在昆虫中，包括蚊子在内，VE是蛋壳中沉积的第一层。它是由卵泡细胞的分泌产物形成的，它的沉积与卵母细胞发育过程中卵黄的积累相吻合。只有靠近卵母细胞的滤泡细胞才产生VE。在断头的雌性动物中，通过灌肠给予血粉并注射皮克剂量的20-羟基蜕皮酮(20-HE)，毛囊细胞合成VE前体并沉积形态正常的VE，而注射生理盐水的对照组不沉积VE。我们得出结论，20-HE以及来自血粉和卵母细胞的因子是蚊子卵泡中VE正常形成所必需的。
Using microsurgical manipulations, hormone applications, and transmission electron microscopy we have investigated the regulation of differentiation of the follicular epithelium and formation of the vitelline envelope (VE) in primary follicles in the ovary of the mosquito, Aedes aegypti. During the first 3 days after eclosion, the primary follicle grows, and cells of the follicular epithelium differentiate, their content of mitochondria, rough endoplasmic reticulum, and Golgi complexes increases significantly. Growth and differentiation of the follicular epithelium appear to be under the control of juvenile hormone (JH), because they are blocked by removal of corpora allata in newly closed adult females and can be restored by either implantation of corpora allata or application of JH III.In insects, including mosquitoes, VE is the first layer of the eggshell to be deposited. It is formed from the secretory products of the follicle cells and its deposition coincides with yolk accumulation by developing oocytes. Only follicle cells adjacent to the oocyte deposit VE. In decapitated females, given a blood meal by enema and injected with picogram doses of 20-hydroxyecdysone (20-HE), follicle cells synthesize the VE precursors and deposit morphologically normal VE, in contrast to saline injected controls which deposit no VE. We conclude that 20-HE, as well as factors originating from the blood meal and the oocyte, are required for the normal formation of VE in the mosquito follicles.