Comparison of the global structure and dynamics of native and unmodified tRNAval.
Comparison of the global structure and dynamics of native and unmodified tRNAval.
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天然和未修饰 tRNAval 的整体结构和动态的比较。
DOI：
10.1021/bi0473399
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发表时间：
2005
期刊：
影响因子：
2.9
通讯作者：
Pardi,Arthur
中科院分区：
文献类型：
--
作者：
Vermeulen,Annaleen;McCallum,ScottA;Pardi,Arthur
利用核磁共振波谱技术研究了转录后修饰对大肠杆菌RNA valRNA结构和动力学的影响。使用NMR化学位移数据和残余偶极偶联来表明，在天然和未修饰的tRNAval中，局部二级和三级结构非常相似。刚体约束的分子动力学计算表明，转录后修饰的tRNA的整体结构是不变的。氘交换NMR实验用于探测天然和未修饰的tRNAval的动力学和灵活性。在两种tRNA中观察到一组相似的缓慢交换（t1/2> 3 min）亚氨基质子，但未修饰的tRNA中最慢交换亚氨基质子的交换速率比天然tRNA快20倍。这些结果表明，tRNAval的动力学和灵活性，但不是局部或全局结构，显着影响转录后修饰。
The effects of post-transcriptional modifications on the structure and dynamics ofEscherichia colitRNAvalwere studied by NMR spectroscopy. NMR chemical shift data and residual dipolar couplings were used to show that the local secondary and tertiary structures are very similar in native and unmodified tRNAval. Rigid body restrained molecular dynamics calculations showed that the global structure of tRNA is unchanged by the post-transcriptional modifications. Deuterium exchange NMR experiments were used to probe the dynamics and flexibility of native and unmodified tRNAval. A similar set of slowly exchanging (t1/2> 3 min) imino protons were observed in both tRNAs, but the rates of exchange for the slowest exchanging imino protons were ∼20 times faster in unmodified than in native tRNA. These results demonstrate that the dynamics and flexibility of tRNAval, but not the local or global structure, are significantly affected by post-transcriptional modifications.