Complete nucleotide sequence and likely recombinatorial origin of bacteriophage T3
Complete nucleotide sequence and likely recombinatorial origin of bacteriophage T3
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DOI：
10.1016/s0022-2836(02)00384-4
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发表时间：
2002-06-21
影响因子：
5.6
通讯作者：
Molineux, IJ
中科院分区：
文献类型：
--
作者：
Pajunen, MI;Elizondo, MR;Molineux, IJ
我们报告了噬菌体T3的全基因组序列（38，208 bp），并与其他完全测序的T7组成员的噬菌体进行了生物信息学比较分析。这种比较表明，T3已经从T7样大肠杆菌噬菌体和耶尔森氏噬菌体之间的重组体进化而来。为了评估这一点，T7和小肠结肠炎耶尔森氏菌血清型O：3噬菌体phiYeO 3 -12之间的重组在体内完成;从该杂交中选择具有T3的许多生物学特性的大肠杆菌噬菌体后代。这代表了第一次实验观察到的重组之间的溶菌，其正常宿主是不同的细菌属。(C)2002爱思唯尔科技有限公司版权所有。
We report the complete genome sequence (38,208 bp) of bacteriophage T3 and provide a bioinformatic comparative analysis with other completely sequenced members of the T7 group of phages. This comparison suggests that T3 has evolved from a recombinant between a T7-like coliphage and a yersiniophage. To assess this, recombination between T7 and the Yersinia enterocolitica serotype O:3 phage phiYeO3-12 was accomplished in vivo; coliphage progeny from this cross were selected that had many biological properties of T3. This represents the first experimentally observed recombination between lytic phages whose normal hosts are different bacterial genera. (C) 2002 Elsevier Science Ltd. All rights reserved.