Toxicity evaluation of high-fluorescent rare-earth metal nanoparticles for bioimaging applications
Toxicity evaluation of high-fluorescent rare-earth metal nanoparticles for bioimaging applications
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DOI：
10.1002/jbm.b.33577
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发表时间：
2017-04-01
影响因子：
3.4
通讯作者：
Palestino, Gabriela
中科院分区：
文献类型：
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作者：
Hernandez-Adame, Luis;Cortez-Espinosa, Nancy;Palestino, Gabriela
纳米发光半导体及其在检测器和对比剂中的生物应用研究是纳米技术的一个新兴领域。在为人类健康开发新的纳米技术时，应深入研究其与生物系统的相互作用。稀土元素用于医疗和工业应用，但其毒性作用尚不清楚。在这项工作中，铽掺杂硫氧化钆纳米粒子（GOSNPs）与人外周血单核细胞（PBMC），人源性巨噬细胞（THP-1），和人宫颈癌细胞（HeLa）的生物相互作用进行了评估。使用水热法合成GOSNP以获得平均尺寸为91 +/-9 nm的单分散纳米颗粒。表征技术表明，六方相的Gd 2 O 2 S：Tb 3+的杂质，并观察到一个强大的绿色发射（emi）=544 nm的Tb 3+产生。使用细胞活力、凋亡、细胞周期进展和免疫应答技术评估GOSNPs的毒性作用。此外，使用卤虫模型来评估体内毒性。结果表明，与HeLa细胞相比，两种类型的细胞中的细胞凋亡对PBMC细胞的敏感性较低。此外，在卤虫体内模型中未观察到毒性作用。此外，GOSNPs显着降低PBMC和HeLa细胞的活化和细胞周期进程，分别。有趣的是，未观察到促炎细胞因子的增加。我们的数据表明，用于生物标记的GOSNPs的荧光应用在原代免疫细胞中是无毒的，并且它们可能具有免疫调节作用。(c)2015 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，105 B：605-615，2017。
Research on nanometer-sized luminescent semiconductors and their biological applications in detectors and contrasting agents is an emergent field in nanotechnology. When new nanosize technologies are developed for human health applications, their interaction with biological systems should be studied in depth. Rare-earth elements are used in medical and industrial applications, but their toxic effects are not known. In this work, the biological interaction between terbium-doped gadolinium oxysulfide nanoparticles (GOSNPs) with human peripheral blood mononuclear cells (PBMC), human-derived macrophages (THP-1), and human cervical carcinoma cell (HeLa) were evaluated. The GOSNPs were synthetized using a hydrothermal method to obtain monodisperse nanoparticles with an average size of 91 +/- 9 nm. Characterization techniques showed the hexagonal phase of the Gd2O2S:Tb3+ free of impurities, and a strong green emission at (emi)=544 nm produced by Tb3+ was observed. Toxic effects of GOSNPs were evaluated using cell viability, apoptosis, cell-cycle progression, and immunological response techniques. In addition, an Artemia model was used to assess the toxicity in vivo. Results indicated cell apoptosis in both types of cells with less sensitivity for PBMC cells compared to HeLa cells. In addition, no toxic effects were observed in the in vivo model of Artemia. Moreover, GOSNPs significantly reduced the activation and cell-cycle progression of PBMC and HeLa cells, respectively. Interestingly, an increase in proinflammatory cytokines was not observed. Our data suggest that fluorescence applications of GOSNPs for biolabeling are not toxic in primary immune cells and they may have an immunomodulatory effect. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 605-615, 2017.