REVERSIBILITY OF PROGRESSION OF THE TRANSFORMED PHENOTYPE IN AD5-TRANSFORMED RAT EMBRYO CELLS
REVERSIBILITY OF PROGRESSION OF THE TRANSFORMED PHENOTYPE IN AD5-TRANSFORMED RAT EMBRYO CELLS
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DOI：
10.1126/science.2581317
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
FISHER, PB
中科院分区：
文献类型：
--
作者：
BABISS, LE;ZIMMER, SG;FISHER, PB
其致癌过程极其复杂，受多种环境和宿主因素的影响。在5型腺病毒(Ad5)转化的大鼠胚胎细胞中，研究了转化表型逐渐发展的机制(称为进展过程)。进展与病毒DBA序列整合模式的重大变化无关。相反，它与整合病毒序列的甲基化增加有关，而不是与Ad5的E1转化基因相对应的序列。单次将进展细胞暴露于脱甲基剂5-氮杂胞苷(Aza)，可稳定地恢复到原始亲本克隆的未进展状态。在琼脂中生长后对细胞的进一步选择允许分离经过氮杂处理的克隆，这些克隆已经恢复了进展的表型。进展显然是一个可逆的过程；可能与一个或多个特定基因甲基化状态的变化有关。
The carcinogenic process is extremely complex and is affected by diverse environmental and host factors. The mechanism for the gradual development of the transformed phenotype (a process termed progression) was studied in type 5 adenovirus (Ad5)-transformed rat embryo cells. Progression was not correlated with major changes in the patterns of integration of viral DBA sequences. Instead, it was associated with an increased methylation of integrated viral sequences other than those corresponding to the E1 transforming gene of Ad5. A single exposure of progressed cells to the demethylating agent 5-azacytidine (Aza) resulted in a stable reversion to the unprogressed state of the original parental clone. A further selection of cells after growth in agar allowed the isolation of Aza-treated clones that had regained the progressed phenotype. Progression evidently is a reversible process; may be associated with changes in the state of methylation of one or more specific genes.