The Apple microR171i-SCARECROW-LIKE PROTEINS26.1 Module Enhances Drought Stress Tolerance by Integrating Ascorbic Acid Metabolism
The Apple microR171i-SCARECROW-LIKE PROTEINS26.1 Module Enhances Drought Stress Tolerance by Integrating Ascorbic Acid Metabolism
复制标题
Apple microR171i-SCARECROW-LIKE PROTEINS26.1 模块通过整合抗坏血酸代谢增强干旱胁迫耐受性
DOI：
10.1104/pp.20.00476
复制
发表时间：
2020
期刊：
影响因子：
7.4
通讯作者：
Li Tianhong
中科院分区：
文献类型：
--
作者：
Wang Yantao;Feng Chen;Zhai Zefeng;Peng Xiang;Wang Yanyan;Sun Yueting;Li Jian;Shen Xiaoshuai;Xiao Yuqin;Zhu Shengjiao;Huang Xuewang;Li Tianhong
Drought stress severely restricts crop yield and quality. Small noncoding RNAs play critical roles in plant growth, development, and stress responses by regulating target gene expression, but their roles in drought stress tolerance in apple (Malus domestica) are poorly understood. Here, we identified various small noncoding RNAs and their targets from the wild apple speciesMalus sieversiivia high-throughput sequencing and degradome analysis. Forty known microRNAs (miRNAs) and eight new small noncoding RNAs were differentially expressed in response to 2 or 4 h of drought stress treatment. We experimentally verified the expression patterns of five selected miRNAs and their targets. We established that one miRNA, mdm-miR171i, specifically targeted and degradedSCARECROW-LIKE PROTEINS26.1(MsSCL26.1) transcripts. Both knockout ofmdm-miR171iand overexpression ofMsSCL26.1improved drought stress tolerance in the cultivated apple line ‘GL-3’ by regulating the expression of antioxidant enzyme genes, especially that ofMONODEHYDROASCORBATE REDUCTASE, which functions in metabolism under drought stress. Transient expression analysis demonstrated that MsSCL26.1 activatesMsMDHARtranscription by positively regulating the activity of theP1region in its promoter. Therefore, themiR171i-SCL26.1module enhances drought stress tolerance in apple by regulating antioxidant gene expression and ascorbic acid metabolism.
