Assays of Sensitivity of Antibiotic-Resistant Bacteria to Hydrogen Peroxide and Measurement of Catalase Activity
Assays of Sensitivity of Antibiotic-Resistant Bacteria to Hydrogen Peroxide and Measurement of Catalase Activity
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DOI：
10.1007/978-1-60327-279-7_7
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Hughes, Diarmaid
中科院分区：
文献类型：
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作者：
Macvanin, Mirjana;Hughes, Diarmaid
细菌和其他利用有氧呼吸的生物一样，会产生和积累破坏DNA、脂肪酸和蛋白质的活性氧物种(ROS)。此外，像肠沙门氏菌这样的细胞内病原体暴露在宿主巨噬细胞产生的氧化爆发中。需氧细菌抵抗氧化应激和清除ROS的相对能力在很大程度上影响着它们在体外和体内的适合性。本文描述了在过氧化氢存在下测量细菌活力和相对适合度的方法。还描述了一种测定过氧化氢酶活性的方法，这是ROS解毒过程中的一个重要部分。
Bacteria, in common with other organisms that take advantage of aerobic respiration, generate and accumulate reactive oxygen species (ROS) that damage DNA, fatty acids, and proteins. In addition, intracellular pathogens like Salmonella enterica are exposed to an oxidate burst produced by host macrophages. The relative ability of aerobically growing bacteria to withstand oxidative stress and eliminate ROS has a large impact of their fitness in vitro and in vivo.Methods arc described here to measure the viability and relative fitness or bacteria in the presence of hydrogen peroxide. A protocol for the determination of catalase activity, an important part of the ROS detoxification process, is also described.