Fusarium keratitis in South India: causative agents, their antifungal susceptibilities and a rapid identification method for the Fusarium solani species complex
Fusarium keratitis in South India: causative agents, their antifungal susceptibilities and a rapid identification method for the Fusarium solani species complex
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DOI：
10.1111/myc.12062
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发表时间：
2013-09-01
期刊：
影响因子：
4.9
通讯作者：
Galgoczy, Laszlo
中科院分区：
文献类型：
--
作者：
Homa, Monika;Shobana, Coimbatore S.;Galgoczy, Laszlo
基于β-微管蛋白（β-TUB）和翻译延伸因子1 α（EF-1 α）基因的部分序列，鉴定了70株来源于人类角膜真菌病的镰刀菌。大多数分离物被证实为F. solani种复合体（75.71%），其次是F. dimerum species complex（8.57%），F. fujikuroi种复合体（8.57%）、F. oxysporum species complex（4.29%）和F.鹿蹄草-木贼种复合体（2.86%）。估计了包括所有70个分离物的组合系统发育树。属于不同种复合体的分离物形成独立的分支。在本研究中，我们还首次报道了F. napiforme从人类角膜真菌病。利用EF-1 α基因上的特异性EcoRI酶切位点，建立了一种快速鉴定F.索拉尼采用微量肉汤稀释法测定了分离菌株对7种抗真菌药物的体外抗菌活性。特比萘芬、那他霉素和阿替霉素B是最有效的药物，其次是伏立康唑。克霉唑、益康唑和伊曲康唑的最低抑菌浓度一般较高（>= 64 lg ml（-1））。用棋盘微量稀释法测定了两种最有效的抗真菌药物纳他霉素和特比萘芬之间的相互作用。两种化合物之间有协同作用（71.8%）或无相互作用（28.2%）。
Seventy Fusarium isolates derived from human keratomycosis were identified based on partial sequences of the beta-tubulin (beta-TUB) and translation elongation factor 1 alpha (EF-1 alpha) genes. Most of the isolates were confirmed as members of the F. solani species complex (75.71%), followed by the F. dimerum species complex (8.57%), the F. fujikuroi species complex (8.57%), the F. oxysporum species complex (4.29%) and the F. incarnatum-equiseti species complex (2.86%). A combined phylogenetic tree was estimated including all the 70 isolates. Isolates belonging to different species complexes formed separate clades. In this study, we also report the first isolation of F. napiforme from human keratomycosis. A new method based on a specific EcoRI restriction site in the EF-1 alpha gene was developed for the rapid identification of F. solani. In vitro antifungal susceptibilities of the isolates to seven antifungals were determined by broth microdilution method. Terbinafine, natamycin and amphotericin B proved to be the most effective drugs, followed by voriconazole. The minimal inhibitory concentrations of clotrimazole, econazole and itraconazole were generally high (>= 64 lg ml(-1)). The interactions between the two most effective antifungals (natamycin and terbinafine) were determined by checkerboard microdilution method. Synergism (71.8%) or no interaction (28.2%) was revealed between the two compounds.