Transcription start regions in the human genome are favored targets for MLV integration
Transcription start regions in the human genome are favored targets for MLV integration
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DOI：
10.1126/science.1083413
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发表时间：
2003-06-13
期刊：
影响因子：
56.9
通讯作者：
Burgess, SM
中科院分区：
文献类型：
--
作者：
Wu, XL;Li, Y;Burgess, SM
促成逆转录病毒整合的因素一直是棘手的，因为过去的研究没有精确定位足够数量的原病毒基因组位点进行重要分析。本研究绘制了903个小鼠白血病病毒（MLV）和379个人类免疫缺陷病毒-1 （HIV-1）在人类基因组中的整合图谱。数据显示，MLV倾向于在转录单元开始附近整合（无论是上游还是下游），而HIV-1倾向于在转录单元的任何地方整合，但不是在转录开始的上游。定义逆转录病毒的不同整合位点将具有重要意义。基因治疗的作用和可能有助于我们理解指导整合过程的因素。
Factors contributing to retroviral integration have been intractable because past studies have not precisely located genomic sites of proviruses in sufficient numbers for significant analysis. In this study, 903 murine leukemia virus (MLV) and 379 human immunodeficiency virus-1 (HIV-1) integrations in the human genome were mapped. The data showed that MLV preferred integration near the start of transcriptional units ( either upstream or downstream) whereas HIV-1 preferred integration anywhere in the transcriptional unit but not upstream of the transcriptional start. Defining different integration site preferences for retroviruses will have important rami. cations for gene therapy and may aid in our understanding of the factors directing the integration process.