Gp91phox-derived Reactive Oxygen Species/Urocortin 2/Corticotropin- releasing Hormone Receptor Type 2 Play an Important Role in Long-term Ultraviolet A Eye Irradiation-induced Photoaging
Gp91phox-derived Reactive Oxygen Species/Urocortin 2/Corticotropin- releasing Hormone Receptor Type 2 Play an Important Role in Long-term Ultraviolet A Eye Irradiation-induced Photoaging
复制标题
Gp91phox 衍生的活性氧/尿皮质素 2/促肾上腺皮质激素释放激素受体 2 型在长期紫外线 A 眼照射引起的光老化中发挥重要作用
DOI：
10.1111/php.12553
复制
发表时间：
2016
影响因子：
3.3
通讯作者：
Y. Yamate
中科院分区：
文献类型：
--
作者：
K. Hiramoto;Y. Yamate
光老化是由长期紫外线A（UVA）眼睛照射引起的。然而，由于UVA眼睛照射引起的皮肤损伤的机制仍然没有很好地理解。在这项研究中，我们使用C57 BL/6 j和gp 91 phox敲除（gp 91 phox −/−）小鼠进行UVA照射的长期影响。小鼠的眼睛或背部皮肤局部暴露于UVA 12个月。UVA照射后，大脑中的活性氧（ROS）、gp 91 phox、促肾上腺皮质激素释放激素（CRH）、尿皮质素2和CRH受体（CRHR）1型和2型水平以及背部皮肤中的肥大细胞类胰蛋白酶和组胺水平均升高。UVA眼部照射后大脑中CRH、尿皮质素2、CRHR 1型和2型的水平也比UVA皮肤照射后增加得更多。此外，UVA眼睛照射小鼠的光老化不会在脑中施用ROS抑制剂后诱导。此外，在gp 91 phox −/−小鼠中，UVA眼睛照射不会诱导光老化。这些结果表明，长期UVA眼部照射导致大脑中gp 91 phox衍生的ROS增加，尿皮质素2和CRHR 2型表达增加，导致光老化;然而，需要进一步的研究来证实这些发现。
Photoaging is induced by long‐term ultraviolet A (UVA) eye irradiation. However, the mechanism of skin damage due to UVA eye irradiation is still not well understood. In this study, we used C57BL/6j and gp91phox knockout (gp91phox−/−) mice for the long‐term effects of UVA irradiation. The eye or dorsal skin of the mice was locally exposed to UVA for 12 months. The reactive oxygen species (ROS), gp91phox, corticotropin‐releasing hormone (CRH), urocortin 2, and CRH receptor (CRHR) type 1 and type 2 levels in the brain and mast cell tryptase and histamine levels in the dorsal skin all increased after UVA irradiation. The levels of CRH, urocortin 2, CRHR type 1 and type 2 in the brain also increased more after UVA eye irradiation than after UVA skin irradiation. Moreover, photoaging of the UVA eye irradiation mice was not induced following the administration of a ROS inhibitor in the brain. In addition, in gp91phox−/−mice, photoaging by UVA eye irradiation was not induced. These results indicate that long‐term UVA eye irradiation led to increased gp91phox‐derived ROS in the brain and the increased expression of urocortin 2 and CRHR type 2, resulting in photoaging; however, further studies are needed to confirm these findings.