Prognostic Significance of Downregulated Expression of Programmed Cell Death 5 in Chondrosarcoma
Prognostic Significance of Downregulated Expression of Programmed Cell Death 5 in Chondrosarcoma
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DOI：
10.1002/jso.21730
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发表时间：
2010-12-01
影响因子：
2.5
通讯作者：
Ma, Qingjun
中科院分区：
文献类型：
--
作者：
Chen, Changbao;Zhou, Hua;Ma, Qingjun
背景与目的：程序性细胞死亡5 （PDCD5）是一种新的细胞凋亡相关基因，PDCD5的调控与肿瘤发生有关。本研究旨在探讨PDCD5在软骨肉瘤中的表达水平，阐明其在软骨肉瘤中的临床意义。方法：采用实时荧光定量PCR和Western blot检测软骨肉瘤及相应非肿瘤组织中PDCD5 mRNA和蛋白水平。分别。采用免疫组化方法检测软骨肉瘤组织中PDCD5蛋白的表达，并分析其与临床病理因素及总生存率的关系。结果：与非肿瘤组织相比，软骨肉瘤组织中PDCD5 mRNA和蛋白水平明显降低。PDCD5蛋白低表达在软骨肉瘤中为61.8%(21/34)，在正常骨中为12.5%(1/8)，在良性软骨肿瘤中为23.5%（4/17）。PDCD5的表达与解剖位置和组织学分级相关。低pdcd5肿瘤患者的生存率低于高pdcd5肿瘤患者。多因素分析显示，PDCD5表达下调是影响总生存的独立预后因素。结论：PDCD5在软骨肉瘤中下调，可能是软骨肉瘤患者总生存率的独立预后因素。中华外科杂志，2010;32(2):838-843。(C)2010 Wiley-Liss, Inc。
Background and Objectives: Programmed Cell Death 5 (PDCD5) is a novel apoptosis-related gene and deregulation of PDCD5 is involved in tumorigenicity. This study was designed to investigate the expression level of PDCD5 and to clarify its clinical significance in chondrosarcoma.Methods: The mRNA and protein levels of PDCD5 in chondrosarcoma and matched corresponding non-tumor tissues were evaluated by real-time PCR and Western blot. respectively. The expression of PDCD5 protein was investigated by immunohistochemistry assays in chondrosarcoma, and its association with clinicopathologic factors and overall survival was also analyzed.Results: The mRNA and protein levels of PDCD5 were significantly decreased in chondrosarcoma compared with corresponding non-tumor tissues. Low expression of PDCD5 protein was 61.8% (21/34) in chondrosarcomas, as compared 12.5% (1/8) in normal bones, as well as compared 23.5% (4/17) in benign cartilage tumors. PDCD5 expression was correlated with anatomical location and histological grade. The survival rate of patients with low-PDCD5 tumors was lower than that of patients with high-PDCD5 tumors. Multivariate analysis revealed that the downregulated expression of PDCD5 was an independent prognostic factor for overall survival.Conclusions: PDCD5 is downregulated in chondrosarcoma and might be an independent prognostic factor for overall survival of chondrosarcoma patients. J. Surg. Oncol, 2010;102:838-843. (C)2010 Wiley-Liss, Inc.