ALTERED GENE-EXPRESSION DURING COLD-ACCLIMATION OF SPINACH
ALTERED GENE-EXPRESSION DURING COLD-ACCLIMATION OF SPINACH
复制标题
DOI：
10.1073/pnas.82.11.3673
复制
发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
BRAMBL, R
中科院分区：
文献类型：
--
作者：
GUY, CL;NIEMI, KJ;BRAMBL, R
菠菜（S。羽衣甘蓝）种植至恒定的5度。C诱导了更大的耐受细胞外冷冻。植物这种冷适应反应的代谢基础尚不清楚。在这项研究中，冷驯化来自改变基因转录的假设进行了测试。植物暴露于低温导致从叶片中提取的可翻译的poly（A）+ RNA群体的快速和稳定的变化，如通过无细胞体外翻译测定所确定的。2个高分子量翻译产物的mRNA种类的最初出现与抗冻性的增加相关。植物冷驯化8天导致mRNA群体的进一步质的变化。在菠菜持续暴露于5 ℃时，至少有四种另外的mRNA种类的浓度增加。C，而其他3种mRNA存在于20 ° C。C生长的叶子减少。还测试了低温诱导的mRNA种类可能编码热休克蛋白的可能性。通过体内标记技术研究了热休克诱导的蛋白质合成，并且在暴露于40 ℃期间合成了至少8种独特的热休克蛋白质。C.然而，大多数多肽暴露于低温诱导，似乎不是热休克蛋白。因此，由低温诱导的mRNA种类的变化是与由高温诱导的变化不同的反应。
Exposure of spinach (S. oleracea) plants to a constant 5.degree. C induced a greater tolerance to extracellular freezing. The metabolic basis of this cold acclimation response in plants is not understood. In this study the hypothesis that cold acclimation derives from altered gene transcription was tested. Exposure of plants to low temperature resulted in a rapid and stable change in the translatable poly(A)+ RNA populations extracted from leaves, as determined by a cell-free in vitro translation assay. The initial appearance of mRNA species for 2 high MW translation products correlated with an increase in freezing tolerance. Cold acclimation of plants for 8 days resulted in further qualitative changes in mRNA populations. At least four additional mRNA species increased in concentration upon continued exposure of spinach to 5.degree. C, whereas 3 other mRNA species present in 20.degree. C-grown leaves decreased. The possibility that the low temperature-induced mRNA species might encode heat shock proteins was also tested. Heat shock-induced protein synthesis by in vivo labeling techniques were studied and at least 8 distinctive heat shock proteins were synthesized during exposure to 40.degree. C. Most polypeptides induced by exposure to low temperature, however, appeared not to be heat shock proteins. Thus, the change in mRNA species induced by low temperature is a separate response from the induced by high temperature.