Characterization of the guinea pig CMV gH/gL/GP129/GP131/GP133 complex in infection and spread
Characterization of the guinea pig CMV gH/gL/GP129/GP131/GP133 complex in infection and spread
复制标题
DOI：
10.1016/j.virol.2013.03.008
复制
发表时间：
2013-06-20
期刊：
影响因子：
3.7
通讯作者：
Feierbach, Becket
中科院分区：
文献类型：
--
作者：
Auerbach, Marcy;Yan, Donghong;Feierbach, Becket
在人巨细胞病毒（HCMV）中，UL 128 - 131 A基因座在细胞嗜性和传播中起着重要作用。在这里，我们报告了豚鼠巨细胞病毒（GPCMV）GP 129 -133位点的完整注释和UL 131 A同源物的发现，命名为GP 133。我们已经发现，与HCMV类似，GP 129 -133蛋白与GPCV糖蛋白gH和gL形成五聚体复合物。此外，我们发现GP 129 -133蛋白在进入中起关键作用，因为GP 129 -133缺失突变体在内皮细胞和成纤维细胞进入中均显示出缺陷。虽然GP 129 -133缺失菌株可以在体外繁殖，但我们发现该缺失不能在体内传播。有趣的是，野生型菌株在体内传播过程中可以自发产生GP 129 -133缺失菌株，这表明该位点的遗传不稳定性。(C)2013 Elsevier Inc. All rights reserved.
In human cytomegalovirus (HCMV), the UL128-131A locus plays an essential role in cellular tropism and spread. Here, we report the complete annotation of the GP129-133 locus from guinea pig cytomegalovirus (GPCMV) and the discovery of the UL131A homolog, named GP133. We have found that similar to HCMV the GP129-133 proteins form a pentamer complex with the GPCMV glycoproteins gH and gL. In addition, we find that the GP129-133 proteins play a critical role in entry as the GP129-133 deletion mutant shows a defect in both endothelial and fibroblast cell entry. Although the GP129-133 deletion strain can propagate in vitro, we find that the deletion fails to spread in vivo. Interestingly, the wildtype strain can spontaneously give rise to the GP129-133 deletion strain during in vivo spread, suggesting genetic instability at this locus. (C) 2013 Elsevier Inc. All rights reserved.